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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09
From 2010.igem.org
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Revision as of 21:07, 23 October 2010
E.coli Fiber Project Notebook
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2010/09/09 Thursday (Naoto)
Experiment:Ligation
member
naoto and NEX
material
- bcsA,B(from A.xylinum) 10µl each
- bcsC,D(from A.xylinum) 10µl each
- bcsA,B(from E.coli) 10µl each
- ligation Mix(nippon gene) 20µl each
procedure
- add material to PCR tube
- incubate (20℃ 10min)
Experiment:Electrophoresis
member
naoto and NEX
material
- ligation products(see above)
we show other material at protocol4
procedure
see protocol3
