Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/09/22 Wednesday (Naoto)== ===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3=== '''member''' naoto and NEX '''mater...)
(→Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli))
 
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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/09/22 Wednesday (Naoto)==
==2010/09/22 Wednesday (Naoto)==
===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
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'''member'''
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:'''Member'''
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naoto and NEX
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:naoto and NEX
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'''material'''
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:'''Material'''
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*bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3
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:*bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3
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you can see other materials at protocol3
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:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
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'''procedure'''
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:'''Procedure'''
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protocol3
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html>
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===Experiment:Insert　check PCR===
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===Experiment:Colony PCR===
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'''member'''
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:'''Member'''
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naoto and NEX
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:naoto and NEX
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'''material'''
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:'''Material'''
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*Colonies of E.coli having bcsA,B(''A.xylinum'')
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:*bcsA,B in E.coli
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you can see other materials at protocol3
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:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
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'''procedure'''
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:'''Procedure'''
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protocol3
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
===Experiment:Electrophoresis of bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
===Experiment:Electrophoresis of bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
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'''member'''
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:'''Member'''
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+
-
naoto and NEX
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-
 
+
-
'''material'''
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-
 
+
-
*PCR production (bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C)
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If you want to know other materials,refer to protocol4
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'''procedure'''
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see protocol4
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-
 
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'''result'''
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Bands of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') could be seen
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:naoto and NEX
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===Experiment:miniprep parts of bcsB,C(''E.coli'')===
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:'''Material'''
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'''member'''
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-
naoto and NEX
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:*PCR production (bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C)
 +
:If you want to know other materials,refer to protocol4
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'''material'''
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:'''Procedure'''
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*bcsB,C(''E.coli'') in pSB1C3
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol4</a></html>
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If you want to know other materials,refer to protocol9
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-
'''procedure'''
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:'''Result'''
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see protocol9
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:Bands of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') could be seen
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===Experiment:Insert　check PCR===
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===Experiment:Colony PCR===
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'''member'''
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:'''Member'''
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naoto and NEX
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:naoto and NEX
-
'''material'''
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:'''Material'''
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*Insert check PCR production (bcsA,B(''A.xylinum''))
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:*PCR production (bcsA,B)
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you can see other materials at protocol3
+
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
-
'''procedure'''
+
:'''Procedure'''
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protocol3
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
-
'''result'''
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:'''Result'''
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a band of No.41(bcsB,''Axylinum'') appeared slightly
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:a band of No.41(bcsB,''Axylinum'') appeared slightly
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===Experiment:Purification of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') with QIAGEN===
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===Experiment:Extraction of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') ===
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'''member'''
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:'''Member'''
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naoto and NEX
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:naoto and NEX
-
'''material'''
+
:'''Material'''
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*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel
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:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel
-
you can see other materials at protocol5
+
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol5</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
see protocol5
+
:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol5</a></html>
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')===
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')===
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'''member'''
+
:'''Member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''Material'''
-
*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')  
+
:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')  
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you can see other materials at protocol6
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:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
-
'''procedure'''
+
:'''Procedure'''
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see protocol6
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
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===Experiment:Subculture ''E.coli'' having bcsB(''A.xylinum'')===
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===Preparation:Make preculture===
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'''member'''
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:'''Member'''
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naoto and NEX
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:naoto and NEX
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'''material'''
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:'''Material'''
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*LB+Cam　Broth
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:*LB+Cam　Broth
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*a colony of E.coli having bcsB(''A.xylinum'')
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:*a colony of E.coli having bcsB(''A.xylinum'')
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you can see other materials at protocol1
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:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>
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'''procedure'''
+
:'''Procedure'''
-
see protocol1
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>

Latest revision as of 14:19, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
you can see other materials at protocol3
Procedure
see protocol3

Experiment:Colony PCR

Member
naoto and NEX
Material
  • bcsA,B in E.coli
you can see other materials at protocol3
Procedure
see protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • PCR production (bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
If you want to know other materials,refer to protocol4
Procedure
see protocol4
Result
Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:Colony PCR

Member
naoto and NEX
Material
  • PCR production (bcsA,B)
you can see other materials at you can see other materials at protocol3
Procedure
see protocol3
Result
a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Extraction of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
you can see other materials at you can see other materials at protocol5
Procedure
see you can see other materials at protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli)
you can see other materials at you can see other materials at protocol7
Procedure
see protocol7

Preparation:Make preculture

Member
naoto and NEX
Material
  • LB+Cam　Broth
  • a colony of E.coli having bcsB(A.xylinum)
you can see other materials at you can see other materials at protocol2
Procedure
see protocol2
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