Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/24

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/9/24 Friday (watachin)==
==2010/9/24 Friday (watachin)==
===Experiment:PCR===
===Experiment:PCR===
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'''Member'''<br />
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:'''Member'''<br />
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easily
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:easily
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'''Materials and Procedure'''
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:'''Materials and Procedure'''
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Direct PCR of bcs genes
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:templete DNA (bcsA,B,C,D)
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
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===Experiment:Insert check PCR===
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===Experiment:Colony PCR===
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'''Member'''<br />
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:'''Member'''<br />
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watachin
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:watachin
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'''Materials and Procedure'''
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:'''Materials and Procedure'''
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Insert check PCR of bcs genes
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
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:E.coli induced bcsA,B,C and D
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Ligation===
===Experiment:Ligation===
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'''Member'''<br />
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:'''Member'''<br />
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easily
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:easily
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'''Materials and Procedure'''
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-
 
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">the protocol(Ligation)</a></html>
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:'''Materials and Procedure'''
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol3</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
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'''Member'''<br />
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:'''Member'''<br />
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watachin
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:watachin
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'''Materials and Procedure'''
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:'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
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'''Member'''<br />
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:'''Member'''<br />
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easily and watachin
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:easily and watachin
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'''Materials and Procedure'''
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:'''Materials and Procedure'''
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
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:'''Result'''
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'''Result'''
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[[Image:2010-09-24-01.jpg]]
[[Image:2010-09-24-01.jpg]]
===Experiment:Transformation===
===Experiment:Transformation===
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'''Member'''<br />
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:'''Member'''<br />
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easily and watachin
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:easily and watachin
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'''Materials and Procedure'''
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 +
:'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>

Latest revision as of 14:33, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/9/24 Friday (watachin)

Experiment:PCR

Member
easily
Materials and Procedure
templete DNA (bcsA,B,C,D)
follow protocol3

Experiment:Colony PCR

Member
watachin
Materials and Procedure
E.coli induced bcsA,B,C and D
follow protocol3

Experiment:Ligation

Member
easily
Materials and Procedure
follow protocol3

Experiment:Digestion

Member
watachin
Materials and Procedure
follow protocol7

Experiment:Electrophoresis

Member
easily and watachin
Materials and Procedure
follow protocol4
Result

2010-09-24-01.jpg

Experiment:Transformation

Member
easily and watachin
Materials and Procedure
follow protocol9
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/24"