Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29

From 2010.igem.org

(Difference between revisions)
(Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))
 
(8 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Tokyo_Metropolitan/Header}}
+
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
 
+
__NOTOC__
==2010/09/29 Wednesday (Naoto)==
==2010/09/29 Wednesday (Naoto)==
===Experiment:Insert check PCR===
===Experiment:Insert check PCR===
Line 10: Line 10:
*bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
*bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
-
If you want to know other materials,refer to protocol3
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
'''procedure'''
'''procedure'''
-
see protocol3
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
Line 27: Line 27:
*bcsQ(''E.coli'')
*bcsQ(''E.coli'')
*T7polymerase(BBa_K145001)
*T7polymerase(BBa_K145001)
-
If you want to know other materials,refer to protocol3
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
'''procedure'''
'''procedure'''
-
see protocol3
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
===Experiment:digestion of bcsB(''E.coli'')===
===Experiment:digestion of bcsB(''E.coli'')===
Line 41: Line 41:
*bcsB(''E.coli'')  
*bcsB(''E.coli'')  
-
If you want to know other materials,refer to protocol6
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
'''procedure'''
'''procedure'''
-
follow to protocol6
+
follow to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
===Experiment:subculture of ''A.xylinum''===
===Experiment:subculture of ''A.xylinum''===
Line 54: Line 54:
'''material'''
'''material'''
-
see protocol1
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
A.xylinum(subcultured at 9/1)
A.xylinum(subcultured at 9/1)
'''procedure'''
'''procedure'''
-
see protocol1  
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
===Experiment:Transformation===
===Experiment:Transformation===
Line 69: Line 69:
*parts of bcsA,B(''A.xylinum'')  
*parts of bcsA,B(''A.xylinum'')  
-
If you want to know other materials,refer to protocol8
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
'''procedure'''
'''procedure'''
-
see protocol8
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
===Experiment:Electrophoresis of insert check PCR===
===Experiment:Electrophoresis of insert check PCR===
Line 83: Line 83:
*PCR production (bcsC,D(''A.xylinum'') )
*PCR production (bcsC,D(''A.xylinum'') )
-
If you want to know other materials,refer to protocol4
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''procedure'''
'''procedure'''
-
see protocol4
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''result'''
'''result'''
Line 102: Line 102:
'''material'''
'''material'''
-
*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli))
+
*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli''))
-
If you want to know other materials,refer to protocol4
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''procedure'''
'''procedure'''
-
see protocol4
+
see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''result'''
'''result'''
-
All bands differed from collect length
+
All bands don't have collect length.

Latest revision as of 16:05, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/29 Wednesday (Naoto)

Experiment:Insert check PCR

member

naoto and NEX

material

  • bcsC,D(A.xylinum) in colonies of E.coli

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase

member

naoto and NEX

material

  • A colony of A.xylinum(for templete DNA)
  • bcsE~G(E.coli)
  • bcsQ(E.coli)
  • T7polymerase(BBa_K145001)

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:digestion of bcsB(E.coli)

member

naoto and easily

material

  • bcsB(E.coli)

If you want to know other materials,refer to protocol6

procedure

follow to protocol6

Experiment:subculture of A.xylinum

member

naoto and easily

material

see protocol1 A.xylinum(subcultured at 9/1)

procedure

see protocol1

Experiment:Transformation

member

naoto and easily

material

  • parts of bcsA,B(A.xylinum)

If you want to know other materials,refer to protocol8

procedure

see protocol8

Experiment:Electrophoresis of insert check PCR

member

naoto

material

  • PCR production (bcsC,D(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly

So we prepared precultures for miniprep

Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)

member

naoto

material

  • PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

All bands don't have collect length.