Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29

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(Difference between revisions)

Latest revision as of 16:05, 27 October 2010

E.coli Fiber Project Notebook

2010/09/29 Wednesday (Naoto)

Experiment:Insert check PCR

member

naoto and NEX

material

bcsC,D(A.xylinum) in colonies of E.coli

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase

member

naoto and NEX

material

A colony of A.xylinum(for templete DNA)
bcsE~G(E.coli)
bcsQ(E.coli)
T7polymerase(BBa_K145001)

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:digestion of bcsB(E.coli)

member

naoto and easily

material

bcsB(E.coli)

If you want to know other materials,refer to protocol6

procedure

follow to protocol6

Experiment:subculture of A.xylinum

member

naoto and easily

material

see protocol1 A.xylinum(subcultured at 9/1)

procedure

see protocol1

Experiment:Transformation

member

naoto and easily

material

parts of bcsA,B(A.xylinum)

If you want to know other materials,refer to protocol8

procedure

see protocol8

Experiment:Electrophoresis of insert check PCR

member

naoto

material

PCR production (bcsC,D(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly

So we prepared precultures for miniprep

Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)

member

naoto

material

PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

All bands don't have collect length.

@@ Line 1: / Line 1: @@
-{{:Team:Tokyo_Metropolitan/Header}}
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
+__NOTOC__
 ==2010/09/29 Wednesday (Naoto)==
 ===Experiment:Insert check PCR===
@@ Line 10: / Line 10: @@
 *bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
-If you want to know other materials,refer to protocol3
+If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 '''procedure'''
-see protocol3
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 ===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
@@ Line 23: / Line 23: @@
 '''material'''
-*A colony of ''A.xylinum''
+*A colony of ''A.xylinum''(for templete DNA)
 *bcsE~G(E.coli)
 *bcsQ(''E.coli'')
 *T7polymerase(BBa_K145001)
-If you want to know other materials,refer to protocol3
+If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 '''procedure'''
-see protocol3
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 ===Experiment:digestion of bcsB(''E.coli'')===
@@ Line 41: / Line 41: @@
 *bcsB(''E.coli'')
-If you want to know other materials,refer to protocol6
+If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
 '''procedure'''
-follow to protocol6
+follow to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
 ===Experiment:subculture of ''A.xylinum''===
@@ Line 54: / Line 54: @@
 '''material'''
-see protocol1
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
 A.xylinum(subcultured at 9/1)
 '''procedure'''
-see protocol1
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
 ===Experiment:Transformation===
@@ Line 69: / Line 69: @@
 *parts of bcsA,B(''A.xylinum'')
-If you want to know other materials,refer to protocol8
+If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
 '''procedure'''
-see protocol8
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
 ===Experiment:Electrophoresis of insert check PCR===
@@ Line 83: / Line 83: @@
 *PCR production (bcsC,D(''A.xylinum'') )
-If you want to know other materials,refer to protocol4
+If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
 '''procedure'''
-see protocol4
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
 '''result'''
@@ Line 95: / Line 95: @@
 So we prepared precultures for miniprep
-===Experiment:Electrophoresis of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli)===
+===Experiment:Electrophoresis of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli'')===
 '''member'''
@@ Line 102: / Line 102: @@
 '''material'''
-*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli))
+*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli''))
-If you want to know other materials,refer to protocol4
+If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
 '''procedure'''
-see protocol4
+see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
 '''result'''
-All bands differed from collect length
+All bands don't have collect length.