Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/09/29 Wednesday (Naoto)==
==2010/09/29 Wednesday (Naoto)==
===Experiment:Insert check PCR===
===Experiment:Insert check PCR===
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*bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
*bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
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If you want to know other materials,refer to protocol3
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If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
'''procedure'''
'''procedure'''
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see protocol3
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
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'''material'''
'''material'''
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*A colony of ''A.xylinum''
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*A colony of ''A.xylinum''(for templete DNA)
*bcsE~G(E.coli)
*bcsE~G(E.coli)
*bcsQ(''E.coli'')
*bcsQ(''E.coli'')
*T7polymerase(BBa_K145001)
*T7polymerase(BBa_K145001)
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If you want to know other materials,refer to protocol3
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
'''procedure'''
'''procedure'''
-
see protocol3
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
===Experiment:digestion of bcsB(''E.coli'')===
===Experiment:digestion of bcsB(''E.coli'')===
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*bcsB(''E.coli'')  
*bcsB(''E.coli'')  
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If you want to know other materials,refer to protocol6
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If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
'''procedure'''
'''procedure'''
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follow to protocol6
+
follow to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
===Experiment:subculture of ''A.xylinum''===
===Experiment:subculture of ''A.xylinum''===
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'''material'''
'''material'''
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see protocol1
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
A.xylinum(subcultured at 9/1)
A.xylinum(subcultured at 9/1)
'''procedure'''
'''procedure'''
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see protocol1  
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol1:Grow_up_a_culture_of_A.xylinum protocol1]
===Experiment:Transformation===
===Experiment:Transformation===
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*parts of bcsA,B(''A.xylinum'')  
*parts of bcsA,B(''A.xylinum'')  
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If you want to know other materials,refer to protocol8
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If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
'''procedure'''
'''procedure'''
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see protocol8
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8]
===Experiment:Electrophoresis of insert check PCR===
===Experiment:Electrophoresis of insert check PCR===
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*PCR production (bcsC,D(''A.xylinum'') )
*PCR production (bcsC,D(''A.xylinum'') )
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If you want to know other materials,refer to protocol4
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If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''procedure'''
'''procedure'''
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see protocol4
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''result'''
'''result'''
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So we prepared precultures for miniprep
So we prepared precultures for miniprep
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===Experiment:Electrophoresis of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli)===
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===Experiment:Electrophoresis of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli'')===
'''member'''
'''member'''
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'''material'''
'''material'''
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*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli))
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*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli''))
-
If you want to know other materials,refer to protocol4
+
If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''procedure'''
'''procedure'''
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see protocol4
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see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
'''result'''
'''result'''
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All bands differed from collect length
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All bands don't have collect length.

Latest revision as of 16:05, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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2010/09/29 Wednesday (Naoto)

Experiment:Insert check PCR

member

naoto and NEX

material

  • bcsC,D(A.xylinum) in colonies of E.coli

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase

member

naoto and NEX

material

  • A colony of A.xylinum(for templete DNA)
  • bcsE~G(E.coli)
  • bcsQ(E.coli)
  • T7polymerase(BBa_K145001)

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:digestion of bcsB(E.coli)

member

naoto and easily

material

  • bcsB(E.coli)

If you want to know other materials,refer to protocol6

procedure

follow to protocol6

Experiment:subculture of A.xylinum

member

naoto and easily

material

see protocol1 A.xylinum(subcultured at 9/1)

procedure

see protocol1

Experiment:Transformation

member

naoto and easily

material

  • parts of bcsA,B(A.xylinum)

If you want to know other materials,refer to protocol8

procedure

see protocol8

Experiment:Electrophoresis of insert check PCR

member

naoto

material

  • PCR production (bcsC,D(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly

So we prepared precultures for miniprep

Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)

member

naoto

material

  • PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

All bands don't have collect length.