Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/18

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/09/18 Sunday (Naoto)==
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==2010/09/18 Saturday (Naoto)==
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
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'''member'''
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:'''Member'''
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naoto and easily
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:naoto and easily
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'''material'''
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:'''Material'''
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*PCR production(pSB1C3)
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:*PCR production(pSB1C3)
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If you want to know other materials,refer to protocol4  
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:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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'''procedure'''
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:'''Procedure'''
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follow to protocol4
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:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
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'''result'''
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:'''Result'''
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Bands didn't appear
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:Bands didn't appear
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===Experiment:Insert check PCR===
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===Experiment:Colony PCR===
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'''member'''
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:'''Member'''
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naoto and easily
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:naoto and easily
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'''material'''
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:'''Material'''
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*Parts of bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli) in colonies of ''E,coli''
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:*Parts of bcsA,B,C,D(''A.xylinum'') bcsA,B,C(''E.coli'')in colonies of ''E,coli''
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If you want to know other materials,refer to protocol3
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:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
-
'''procedure'''
+
:'''Procedure'''
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follow to protocol3
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:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
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===Experiment:Direct PCR===
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===Experiment:PCR===
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'''member'''
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:'''Member'''
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naoto and easily
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:naoto and easily
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'''material'''
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:'''Material'''
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*bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3
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:*bcsA,B,C,D(''A.xylinum''),pSB1C3
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If you want to know other materials,refer to protocol3
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
-
'''procedure'''
+
:'''Procedure'''
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follow to protocol3
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:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
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===Experiment:Electrophoresis of bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3===
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===Experiment:Electrophoresis of bcsA,B,C,D(''A.xylinum''),pSB1C3===
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'''member'''
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:'''Member'''
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naoto and easily
+
:naoto and easily
-
'''material'''
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:'''Material'''
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*PCR production(bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3)
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:*PCR production(bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli),pSB1C3)
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If you want to know other materials,refer to protocol4  
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:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
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follow to protocol4
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:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
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'''result'''
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:'''Result'''
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Only band of bcsA(''E.coli'') could be seen
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:Only band of bcsA(''E.coli'') could be seen
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===Experiment:Purification bcsA(''E.coli'')===
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===Experiment:DNA Extraction from agarose gel===
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'''member'''
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:'''Member'''
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naoto and easily
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:naoto and easily
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'''material'''
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:'''Material'''
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*bcsA(''E.coli'') in agarose gel
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:*bcsA(''E.coli'') in agarose gel
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If you want to know other materials,refer to protocol5
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:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol5</a></html>
-
'''procedure'''
+
:'''Procedure'''
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follow to protocol5
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:follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol5</a></html>
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===Experiment:Electrophoresis of insert check PCR===
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===Experiment:Electrophoresis of Colony PCR===
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'''member'''
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:'''Member'''
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naoto and easily
+
:naoto and easily
-
'''material'''
+
:'''Material'''
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*PCR production (parts of bcsA,B,C,D(''A.xylinum''),bcsA,B,C(E.coli) in colonies of ''E,coli'')
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:*PCR production (parts of bcsA,B,C,D(''A.xylinum'') and bcsA(''E.coli'')in colonies of ''E,coli'')
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If you want to know other materials,refer to protocol4
+
:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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follow to protocol4
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:'''Result'''
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'''result'''
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:Bands of bcsB,C(''A.xylinum'') appreared
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:*1~8  bcsA(''A.xylinum'')
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:*9~12  bcsB(''A.xylinum'')
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:*13    bcsC(''A.xylinum'')
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:*14~15 bcsD(''A.xylinum'')
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:*16~20 bcsA(''E.coli'')
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Bands of bcsB,C(''A.xylinum'') appreared
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<br/>
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*1~8  bcsA(''A.xylinum'')
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*9~12  bcsB(''A.xylinum'')
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*13    bcsC(''A.xylinum'')
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*14~15 bcsD(''A.xylinum'')
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*16~20 bcsA(''E.coli'')
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Latest revision as of 07:11, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
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31

2010/09/18 Saturday (Naoto)

Experiment:Electrophoresis

Member
naoto and easily
Material
  • PCR production(pSB1C3)
If you want to know other materials,refer to protocol4
Procedure
follow to protocol3
Result
Bands didn't appear

Experiment:Colony PCR

Member
naoto and easily
Material
  • Parts of bcsA,B,C,D(A.xylinum) bcsA,B,C(E.coli)in colonies of E,coli
If you want to know other materials,refer to protocol3
Procedure
follow to protocol3

Experiment:PCR

Member
naoto and easily
Material
  • bcsA,B,C,D(A.xylinum),pSB1C3
If you want to know other materials,refer to protocol3
Procedure
follow to protocol3

Experiment:Electrophoresis of bcsA,B,C,D(A.xylinum),pSB1C3

Member
naoto and easily
Material
  • PCR production(bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3)
If you want to know other materials,refer to protocol4
Procedure
follow to protocol3
Result
Only band of bcsA(E.coli) could be seen

Experiment:DNA Extraction from agarose gel

Member
naoto and easily
Material
  • bcsA(E.coli) in agarose gel
If you want to know other materials,refer to protocol5
Procedure
follow to protocol5

Experiment:Electrophoresis of Colony PCR

Member
naoto and easily
Material
  • PCR production (parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)in colonies of E,coli)
If you want to know other materials,refer to protocol4
Procedure
refer to protocol4
Result
Bands of bcsB,C(A.xylinum) appreared
  • 1~8 bcsA(A.xylinum)
  • 9~12 bcsB(A.xylinum)
  • 13 bcsC(A.xylinum)
  • 14~15 bcsD(A.xylinum)
  • 16~20 bcsA(E.coli)

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/18"