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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06
From 2010.igem.org
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===1:PCR=== | ===1:PCR=== | ||
<Member><br /> | <Member><br /> | ||
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<Sample><br /> | <Sample><br /> | ||
| - | :*1 | + | :*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | :*2 | + | :*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | :*5 | + | :*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
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:*2(BBa_JM109) 71.0,71.5,72.0℃ | :*2(BBa_JM109) 71.0,71.5,72.0℃ | ||
:*5(BBa_K208017) 70.0,70.5,71.0℃ | :*5(BBa_K208017) 70.0,70.5,71.0℃ | ||
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===2:DNA extraction=== | ===2:DNA extraction=== | ||
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<Samlpe><br /> | <Samlpe><br /> | ||
PCR products. | PCR products. | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
<Protocol><br /> | <Protocol><br /> | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
<Protocol> | <Protocol> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | ||
Latest revision as of 18:14, 24 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/09/06
1:PCR
<Member>
Hitomi,nito
<Sample>
<Protocol>
See Protocol 2
Tubes(Temperature in annealing)
- 1(BBa_K208017) 71.5,72.0,72.5℃
- 2(BBa_JM109) 71.0,71.5,72.0℃
- 5(BBa_K208017) 70.0,70.5,71.0℃
2:DNA extraction
<Member>
Hitomi
<Protocol>
See Protocol 4
3:Electrophoresis
<Samlpe>
PCR products.
<Protocol>
See Protocol 8
<Result>
.JPG)
4:Ligation
<Membber>
nito
<Sample>
<Protocol>
See Protocol 3
