E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/09/06 1.1 1:PCR 1.2 2:DNA extraction 1.3 3:Electrophoresis 1.4 4:Ligation

2010/09/06

1:PCR

<Member>
Hitomi,nito

<Sample>

• 1
• 2
• 5

<Protocol>
See Protocol 2

Tubes(Temperature in annealing)

• 1(BBa_K208017) 71.5,72.0,72.5℃
• 2(BBa_JM109) 71.0,71.5,72.0℃
• 5(BBa_K208017) 70.0,70.5,71.0℃

2:DNA extraction

<Member>
Hitomi

<Protocol>
See Protocol 4

3:Electrophoresis

<Samlpe>
PCR products.

• 1
• 2
• 5
• 7
• 8

<Protocol>
See Protocol 8

<Result>

4:Ligation

<Membber>
nito

<Sample>

• 7
• 8

<Protocol> See Protocol 3