Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/31

From 2010.igem.org

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=2010/08/31=
=2010/08/31=
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＜Sample＞<br />
＜Sample＞<br />
PCR products.
PCR products.
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*1 (8/25)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1 ] (8/25)   
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*3 (8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3 ] (8/26)   
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*4 (8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4 ] (8/26)   
-
*5 (8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5 ] (8/26)   
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*7(8/30)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7 ] (8/30)
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*8(8/30)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8 ] (8/30)
   
   
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＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
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*1L(8/25)  8μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L ](8/25)  8μl
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*2L(8/26)  8μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L ](8/26)  8μl
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*4H(8/26)  16μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H ](8/26)  16μl
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*5H(8/26)  16μl
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H ](8/26)  16μl
・Digest enzyme
・Digest enzyme
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＜Sample＞<br />
＜Sample＞<br />
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*1+2(8/31)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2 ](8/31)   
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*4+5(8/31)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5 ](8/31)   
(after digestion)
(after digestion)
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And cut off gels included DNA.
And cut off gels included DNA.
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＜Result＞<br />
 
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[[Image:Matsuura_2010-08-31_17hr_16min.tif]]
 
==6：DNA extraction==
==6：DNA extraction==
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＜Sample＞<br />
＜Sample＞<br />
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*1+2(8/31)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2 ](8/31)   
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*4+5(8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5 ](8/31)
(After electrophoresis)
(After electrophoresis)
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＜Protocol＞<br />
＜Protocol＞<br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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==8：DNA Ligation ==
==8：DNA Ligation ==
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＜Sample＞<br />
＜Sample＞<br />
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*1L(8/25)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L ](8/25)   
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*2L(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26)   
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*4H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26)   
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*5H(8/26)   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26)   
(after digestion)
(after digestion)
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We ligated 1 and 2, 4 and 5.
We ligated 1 and 2, 4 and 5.
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==9：Electrophoresis==
==9：Electrophoresis==
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＜Sample＞<br />
＜Sample＞<br />
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*1+2(8/31)   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2](8/31)   
-
*4+5(8/31)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5](8/31)
(After ligation)
(After ligation)
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＜Result＞<br />
＜Result＞<br />
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[[Image:Matsuura_2010-08-31_21hr_56min.tif]]
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[[Image:Matsuura_2010-08-06_20hr_02min_(14).JPG]]

Latest revision as of 18:13, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/08/31

1：Electrophoresis

＜Member＞
Hitomi


＜Sample＞
PCR products.


＜Protocol＞
SeeProtocol 8


＜Result＞ Matsuura 2010-08-06 20hr 02min (10).JPG

2：PCR

＜Member＞
nito


＜Sample＞
・E-coli (having BBa_I13521 plasmid)

・E-coli (having BBa_K208017 plasmid)

・E-coli (having BBa_I732901 plasmid)


＜Protocol＞
See Protocol 2

・Tube (temperature in annealing)

  1. Promoter~signal (72.0℃)
  2. cyaA (71.5)
  3. mRFP~Terminator (69.0)
  4. Promoter (70.0)
  5. RBS~signal (70.0)
  6. lacZ (63.5)
  7. Terminator (67.5)
  8. CRP (72.5)

All tubes ×3. Total 24 tubes.


3：DNA Digestion

＜Member＞
Mariko, Hitomi


＜Sample, Materials＞
・PCR productions

  • 1L (8/25) 8μl
  • 2L (8/26) 8μl
  • 4H (8/26) 16μl
  • 5H (8/26) 16μl

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


＜Protocol＞
See Protocol 9

4：Electrophoresis

＜Member＞
nito, Hitomi


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (11).JPG

5：Electrophoresis

＜Member＞
Mariko, nito


＜Sample＞

(after digestion)


＜Protocol＞
SeeProtocol 8

And cut off gels included DNA.

6：DNA extraction

＜Member＞
nito


＜Sample＞
・PCR productions (8/31)


＜Protocol＞
SeeProtocol 4


7：DNA extraction from gels

＜Member＞
nito


＜Sample＞

(After electrophoresis)


＜Protocol＞
SeeProtocol 4

8：DNA Ligation

＜Member＞
nito


＜Sample＞

(after digestion)


＜Protocol＞
See Protocol 3

We ligated 1 and 2, 4 and 5.

9：Electrophoresis

＜Member＞
nito


＜Sample＞

(After ligation)


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-06 20hr 02min (14).JPG

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/08/31"