Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/31

From 2010.igem.org


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
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1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/08/31

1:Electrophoresis

<Member>
Hitomi


<Sample>
PCR products.


<Protocol>
SeeProtocol 8


<Result> Matsuura 2010-08-06 20hr 02min (10).JPG

2:PCR

<Member>
nito


<Sample>
・E-coli (having BBa_I13521 plasmid)

・E-coli (having BBa_K208017 plasmid)

・E-coli (having BBa_I732901 plasmid)


<Protocol>
See Protocol 2

・Tube (temperature in annealing)

  1. Promoter~signal (72.0℃)
  2. cyaA (71.5)
  3. mRFP~Terminator (69.0)
  4. Promoter (70.0)
  5. RBS~signal (70.0)
  6. lacZ (63.5)
  7. Terminator (67.5)
  8. CRP (72.5)

All tubes ×3. Total 24 tubes.


3:DNA Digestion

<Member>
Mariko, Hitomi


<Sample, Materials>
・PCR productions

  • 1L (8/25) 8μl
  • 2L (8/26) 8μl
  • 4H (8/26) 16μl
  • 5H (8/26) 16μl

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


<Protocol>
See Protocol 9

4:Electrophoresis

<Member>
nito, Hitomi


<Sample>
・PCR products


<Protocol>
SeeProtocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (11).JPG

5:Electrophoresis

<Member>
Mariko, nito


<Sample>

(after digestion)


<Protocol>
SeeProtocol 8

And cut off gels included DNA.

6:DNA extraction

<Member>
nito


<Sample>
・PCR productions (8/31)


<Protocol>
SeeProtocol 4


7:DNA extraction from gels

<Member>
nito


<Sample>

(After electrophoresis)


<Protocol>
SeeProtocol 4

8:DNA Ligation

<Member>
nito


<Sample>

(after digestion)


<Protocol>
See Protocol 3

We ligated 1 and 2, 4 and 5.

9:Electrophoresis

<Member>
nito


<Sample>

(After ligation)


<Protocol>
SeeProtocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (14).JPG