Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06
From 2010.igem.org
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+ | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} | ||
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=2010/09/06= | =2010/09/06= | ||
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+ | ===1:PCR=== | ||
+ | <Member><br /> | ||
Hitomi,nito | Hitomi,nito | ||
- | <Sample> | + | <Sample><br /> |
- | :*1 | + | :*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
- | :*2 | + | :*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
- | :*5 | + | :*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
- | <Protocol> | + | <Protocol><br /> |
- | + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | |
- | See | + | |
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- | + | ||
- | + | ||
Tubes(Temperature in annealing) | Tubes(Temperature in annealing) | ||
Line 24: | Line 22: | ||
:*5(BBa_K208017) 70.0,70.5,71.0℃ | :*5(BBa_K208017) 70.0,70.5,71.0℃ | ||
- | ===2: | + | ===2:DNA extraction=== |
- | + | <Member><br /> | |
- | <Member> | + | |
- | + | ||
Hitomi | Hitomi | ||
- | <Protocol> | + | <Protocol><br /> |
+ | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
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===3:Electrophoresis=== | ===3:Electrophoresis=== | ||
- | <Samlpe> | + | <Samlpe><br /> |
- | + | ||
PCR products. | PCR products. | ||
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] | |
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] | |
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] | |
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] | |
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] | |
- | + | <Protocol><br /> | |
- | <Protocol> | + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] |
- | + | ||
- | See | + | |
- | + | ||
- | + | ||
<Result> | <Result> | ||
- | [[image:Matsuura_2010- | + | [[image:Matsuura_2010-08-06_20hr_02min_(16).JPG]] |
===4:Ligation=== | ===4:Ligation=== | ||
- | + | <Membber><br /> | |
- | + | ||
- | <Membber> | + | |
- | + | ||
nito | nito | ||
- | <Sample> | + | <Sample><br /> |
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] | |
- | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] | |
- | + | ||
<Protocol> | <Protocol> | ||
- | + | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | |
- | See | + |
Latest revision as of 18:14, 24 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/09/06
1:PCR
<Member>
Hitomi,nito
<Sample>
<Protocol>
See Protocol 2
Tubes(Temperature in annealing)
- 1(BBa_K208017) 71.5,72.0,72.5℃
- 2(BBa_JM109) 71.0,71.5,72.0℃
- 5(BBa_K208017) 70.0,70.5,71.0℃
2:DNA extraction
<Member>
Hitomi
<Protocol>
See Protocol 4
3:Electrophoresis
<Samlpe>
PCR products.
<Protocol>
See Protocol 8
<Result>
4:Ligation
<Membber>
nito
<Sample>
<Protocol>
See Protocol 3