Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/13

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/9/13 Monday (Naoto)== ===Experiment:direct PCR=== '''member''' naoto '''material''' *bcsA,B,C(''A.xylinum'')and bcsB(''E.coli'') 0.5µl If you...)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Tokyo_Metropolitan/Header}}
+
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
==2010/9/13 Monday (Naoto)==
+
__NOTOC__
-
===Experiment:direct PCR===
+
==2010/09/13 Monday (Naoto)==
-
'''member'''
+
===Experiment:PCR===
 +
:'''Member'''
-
naoto
+
:naoto
-
'''material'''
+
:'''Material'''
-
*bcsA,B,C(''A.xylinum'')and bcsB(''E.coli'') 0.5µl
+
:*bcsA,B,C
-
If you want to know other materials, see protocol3
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-
'''procedure'''
+
'''Procedure'''
-
follow to protocol3
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
===Experiment:electrophoresis===
===Experiment:electrophoresis===
-
'''member'''
+
:'''Member'''
-
naoto
+
:naoto
-
'''material'''
+
:'''Material'''
-
*PCR production(see above)
+
:*PCR production(see above)
-
If you want to know other materials, see protocol4
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol4
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''result'''
+
:'''result'''
-
bands of bcsA,C(''A.xylinum'')and bcsB(''E.coli'')is appear clearly
+
:bands of bcsA,C(''A.xylinum'')and appeared clearly
-
===Experiment:Purification from agarose gel with QIAGEN===
+
===Experiment:DNA Extraction from agarose gel===
-
'''member'''
+
:'''Member'''
-
naoto
+
:naoto
-
'''material'''
+
:'''Material'''
-
*bcsA,C(''A.xylinum'')and bcsB(''E.coli'') in agarose gel
+
:*bcsA,C(''A.xylinum'')in agarose gel
-
If you want to know other materials, see protocol5
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
follow to protocol5
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
 
 +
<br/>

Latest revision as of 06:51, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/13 Monday (Naoto)

Experiment:PCR

Member
naoto
Material
  • bcsA,B,C
If you want to know other materials, see protocol3

Procedure

follow protocol3

Experiment:electrophoresis

Member
naoto
Material
  • PCR production(see above)
If you want to know other materials, see protocol4
Procedure
follow protocol4
result
bands of bcsA,C(A.xylinum)and appeared clearly

Experiment:DNA Extraction from agarose gel

Member
naoto
Material
  • bcsA,C(A.xylinum)in agarose gel
If you want to know other materials, see protocol5
Procedure
follow protocol5