Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24
From 2010.igem.org
(Difference between revisions)
(New page: =August 24th (bambi75)= ==Agarose Gels Electro-phoresis== ===member=== NEX , bambi75 and watachin ===Materials,Procedure=== Same protocol on August 16th. PCR Product of insert are bcsA...) |
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- | = | + | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} |
+ | __NOTOC__ | ||
+ | ==2010/08/24 Tuesday(bambi75)== | ||
+ | ===Experiment:Agarose Gels Electrophoresis=== | ||
- | + | :'''member''' | |
- | + | :NEX , bambi75 and watachin | |
- | NEX , bambi75 and watachin | + | |
- | + | :'''Materials,Procedure''' | |
- | + | ||
- | + | ||
+ | :Same protocol on August 16th. | ||
+ | :PCR Product of insert are bcsA and bcsC. | ||
- | == | + | ===Experiment:PCR=== |
- | + | :'''Member''' | |
- | + | ||
- | + | :NEX , bambi75 and watachin | |
- | + | ||
+ | :'''Material''' | ||
- | + | :*sterilized water 213μl | |
+ | :*Ex taq buffer 30μl | ||
+ | :*dNTP 24μl | ||
+ | :*Ex taq 3μl | ||
+ | :*bcsA sense 15μl | ||
+ | :*bcsA antisense 15μl | ||
- | + | :'''Procedure''' | |
- | + | ||
- | = | + | :Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | === | + | ===Experiment:Restriction enzyme digestion=== |
- | + | ||
+ | :'''Member''' | ||
- | + | :Same above | |
- | + | :'''Materials''' | |
- | + | ||
- | + | :*PCR production | |
- | *PCR | + | :*M buffer 30μl |
- | * | + | :*BSA 30μl |
- | *BSA 30μl | + | :*Xba1 6μl |
- | *Xba1 6μl | + | :*Spe1 6μl |
- | * | + | |
- | + | :'''Procedure''' | |
- | + | ||
+ | :Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol7</a></html> | ||
- | ==Transformation== | + | ===Experiment:Transformation=== |
- | + | :'''Member''' | |
- | + | ||
- | + | :Same above | |
- | + | ||
+ | :'''Materials , Procedure''' | ||
+ | |||
+ | :Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html> | ||
+ | |||
+ | <br/> |
Latest revision as of 21:45, 26 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/08/24 Tuesday(bambi75)
Experiment:Agarose Gels Electrophoresis
- member
- NEX , bambi75 and watachin
- Materials,Procedure
- Same protocol on August 16th.
- PCR Product of insert are bcsA and bcsC.
Experiment:PCR
- Member
- NEX , bambi75 and watachin
- Material
- sterilized water 213μl
- Ex taq buffer 30μl
- dNTP 24μl
- Ex taq 3μl
- bcsA sense 15μl
- bcsA antisense 15μl
- Procedure
- Please see protocol3
Experiment:Restriction enzyme digestion
- Member
- Same above
- Materials
- PCR production
- M buffer 30μl
- BSA 30μl
- Xba1 6μl
- Spe1 6μl
- Procedure
- Please see protocol7
Experiment:Transformation
- Member
- Same above
- Materials , Procedure
- Please refer to protocol9