Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/11

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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==2010/8/11 Wednesday (Naoto)==
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==2010/08/11 Wednesday (Naoto)==
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===Experiment:Grow up a culture of ''A.xylinum''===
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===Experiment:Grow up a culture of A.xylinum===
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'''Member'''<br />
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:'''Member'''
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easiry and naoto
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'''Materials'''
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:easily and naoto
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*''A.xylinum'' JCM strain 7644
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*media for A.xylinum(Open Wet Ware recommended)
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*media for A.xylinum(JCM recommended) 100ml
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glucoce 10g
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yeast extract 1g
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CaCO3 3g
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DW 100ml
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(add agar 1.5g for plates)
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*recovery solution for ''A.xylinum'' 100ml
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peptone 0.5g
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yeast extract 0.3g
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DW 100ml
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'''Procedure'''
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:'''Material'''
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#prepared media (plates of Open Wet Ware recommended media were made yesterday)
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#autoclaved to sterilize media(121°C 20minute)
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#added recovery solution to an ampule which had ''A.xylinum''
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#streaked ''A.xylinum'' onto plates or in media
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#incubated at 26°C
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:*''A.xylinum'' JCM 7644 strain
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:*Acetobacter medium (see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_A.xylinum">protocol1</a></html>)
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:*recovery solution for A.xylinum
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:'''Procedure'''
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===Experiment:Grow up a culture of ''E.coli''===
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:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_A.xylinum">protocol1</a></html>
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'''Member'''<br />
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NEX,Bambi75 and watachin
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'''Materials'''<br />
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===Experiment:Grow up a culture of E.coli===
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LB plates
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:'''Member'''
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'''Procedure'''
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:NEX,Bambi75 and watachin
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#streaked ''E.coli'' onto plates
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#incubated at 37°C
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:'''Material'''
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:*''E.coli'' K12 JM109
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:*LB medium (see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_E.coli">protocol2</a></html>)
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:'''Procedure'''
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:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_E.coli">protocol2</a></html>
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<br/>

Latest revision as of 21:25, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/08/11 Wednesday (Naoto)

Experiment:Grow up a culture of A.xylinum

Member
easily and naoto
Material
  • A.xylinum JCM 7644 strain
  • Acetobacter medium (see protocol1)
  • recovery solution for A.xylinum
Procedure
refer to protocol1

Experiment:Grow up a culture of E.coli

Member
NEX,Bambi75 and watachin
Material
Procedure
refer to protocol2