"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25
From 2010.igem.org
(Difference between revisions)
(→Experiment:DNA purification with silica gel) |
(→Experiment:Ligatiom) |
||
| (One intermediate revision not shown) | |||
| Line 51: | Line 51: | ||
:'''Procedure'''<br /> | :'''Procedure'''<br /> | ||
| - | :See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel"> | + | :See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel">protocol6</a></html> |
===Experiment:Ligatiom=== | ===Experiment:Ligatiom=== | ||
| Line 64: | Line 64: | ||
:'''Procedure'''<br/> | :'''Procedure'''<br/> | ||
:Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html> | :Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html> | ||
| + | |||
| + | <br/> | ||
Latest revision as of 14:57, 27 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/09/25 Saturday(NEX)
Experiment:PCR
- Member
- easily
- Material
- templete DNA:pSB1C3
- sense primers of pSB1C3
- antisense primers of pSB1C3
- Procedure
- Refer to protocol3
Experiment:Ligatiom
- Member
- easily
- Material
- bcsA from E.coli 20μl
- bcsB from E.coli 20μl
- 2×ligation mix 40μl
- Procedure
- Follow to protocol7
Experiment:Agarose gel electrophoresis
- Member
- easily
- Material
- Today's PCR productions
- Today's ligation productions
- Agarose gel
- Procedure
- Refer to protocol4
- Result
- pSB1C3 were appeared
Experiment:DNA purification with silica gel
- Member
- easily
- Materials
- pSB1C3
- Procedure
- See protocol6
Experiment:Ligatiom
- Member
- easily
- Material
- pSB1C3 3μl
- bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
- 2×ligation mix 6μl
- Procedure
- Follow to protocol7
