Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

From 2010.igem.org

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:'''Materials and Procedure'''
:'''Materials and Procedure'''
-
:Colony PCR of bcs genes
+
:E.coli introduced bcsA,B,C,D
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Plating===
===Experiment:Plating===
Line 20: Line 20:
:plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
:plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
-
===Experiment:Insert check PCR===
+
===Experiment:Colony PCR===
:'''Member'''<br />
:'''Member'''<br />
:naoto
:naoto
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:Colony PCR production of bcs genes
:Colony PCR production of bcs genes
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Insert check Electrophoresis===
===Experiment:Insert check Electrophoresis===
Line 36: Line 36:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
:'''Result'''
:'''Result'''
Line 65: Line 65:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
:*Colony PCR production
:*Colony PCR production
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
:'''Result'''
:'''Result'''
Line 77: Line 77:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
Line 85: Line 85:
:'''Materials and Procedure'''
:'''Materials and Procedure'''
-
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
:'''Result'''
:'''Result'''

Latest revision as of 14:27, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
SUNMONTUEWEDTHUFRISAT
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2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto
Materials and Procedure
E.coli introduced bcsA,B,C,D
follow protocol3

Experiment:Plating

Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Colony PCR

Member
naoto
Materials and Procedure
Colony PCR production of bcs genes
follow protocol3

Experiment:Insert check Electrophoresis

Member
watachin
Materials and Procedure
follow protocol5
Result

2010-09-23-01.jpg 2010-09-23-02.jpg

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
  • Colony PCR production
follow protocol4
Result

2010-09-23-03.jpg

Experiment:Transformation

Member
watachin
Materials and Procedure
follow protocol9

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
follow protocol4
Result

2010-09-23-04.jpg

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5
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