Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

From 2010.igem.org

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(→Experiment:Colony PCR)
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:'''Procedure'''
:'''Procedure'''
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:<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html>
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html>
===Experiment:Colony PCR===
===Experiment:Colony PCR===
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:a band of No.41(bcsB,''Axylinum'') appeared slightly
:a band of No.41(bcsB,''Axylinum'') appeared slightly
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===Experiment:Purification of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') with QIAGEN===
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===Experiment:Extraction of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') ===
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:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel
:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel
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:you can see other materials at protocol5
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:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol5</a></html>
:'''Procedure'''
:'''Procedure'''
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:see protocol5
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:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol5</a></html>
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')===
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')===
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:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')  
:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')  
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
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:'''Procedure'''
:'''Procedure'''
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:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
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===Preparation:Make preculture===
===Preparation:Make preculture===
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:*a colony of E.coli having bcsB(''A.xylinum'')
:*a colony of E.coli having bcsB(''A.xylinum'')
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>
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:'''Procedure'''
:'''Procedure'''
-
:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>

Latest revision as of 14:19, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
you can see other materials at protocol3
Procedure
see protocol3

Experiment:Colony PCR

Member
naoto and NEX
Material
  • bcsA,B in E.coli
you can see other materials at protocol3
Procedure
see protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • PCR production (bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
If you want to know other materials,refer to protocol4
Procedure
see protocol4
Result
Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:Colony PCR

Member
naoto and NEX
Material
  • PCR production (bcsA,B)
you can see other materials at you can see other materials at protocol3
Procedure
see protocol3
Result
a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Extraction of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
you can see other materials at you can see other materials at protocol5
Procedure
see you can see other materials at protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli)
you can see other materials at you can see other materials at protocol7
Procedure
see protocol7

Preparation:Make preculture

Member
naoto and NEX
Material
  • LB+Cam　Broth
  • a colony of E.coli having bcsB(A.xylinum)
you can see other materials at you can see other materials at protocol2
Procedure
see protocol2
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