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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22
From 2010.igem.org
(Difference between revisions)
(→Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3) |
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:'''Procedure''' | :'''Procedure''' | ||
| - | :<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html> |
===Experiment:Colony PCR=== | ===Experiment:Colony PCR=== | ||
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:'''Procedure''' | :'''Procedure''' | ||
| - | : | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html> |
===Experiment:Electrophoresis of bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3=== | ===Experiment:Electrophoresis of bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3=== | ||
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:'''Procedure''' | :'''Procedure''' | ||
| - | : | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html> |
:'''Result''' | :'''Result''' | ||
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:a band of No.41(bcsB,''Axylinum'') appeared slightly | :a band of No.41(bcsB,''Axylinum'') appeared slightly | ||
| - | ===Experiment: | + | ===Experiment:Extraction of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') === |
:'''Member''' | :'''Member''' | ||
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:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel | :*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel | ||
| - | :you can see other materials at protocol5 | + | :you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol5</a></html> |
:'''Procedure''' | :'''Procedure''' | ||
| - | :see protocol5 | + | :see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol5</a></html> |
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')=== | ===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')=== | ||
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:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') | :*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') | ||
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html> | :you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html> | ||
| - | |||
:'''Procedure''' | :'''Procedure''' | ||
| - | :see | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html> |
| - | + | ||
===Preparation:Make preculture=== | ===Preparation:Make preculture=== | ||
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:*a colony of E.coli having bcsB(''A.xylinum'') | :*a colony of E.coli having bcsB(''A.xylinum'') | ||
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html> | :you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html> | ||
| - | |||
:'''Procedure''' | :'''Procedure''' | ||
| - | :see | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html> |
Latest revision as of 14:19, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/22 Wednesday (Naoto)
Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
- Member
- naoto and NEX
- Material
- bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
- you can see other materials at protocol3
- Procedure
- see protocol3
Experiment:Colony PCR
- Member
- naoto and NEX
- Material
- bcsA,B in E.coli
- you can see other materials at protocol3
- Procedure
- see protocol3
Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
- Member
- naoto and NEX
- Material
- PCR production (bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
- If you want to know other materials,refer to protocol4
- Procedure
- see protocol4
- Result
- Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen
Experiment:Colony PCR
- Member
- naoto and NEX
- Material
- PCR production (bcsA,B)
- you can see other materials at you can see other materials at protocol3
- Procedure
- see protocol3
- Result
- a band of No.41(bcsB,Axylinum) appeared slightly
Experiment:Extraction of bcsB(A.xylinum) and bcsA,B,C(E.coli)
- Member
- naoto and NEX
- Material
- bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
- you can see other materials at you can see other materials at protocol5
- Procedure
- see you can see other materials at protocol5
Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)
- Member
- naoto and NEX
- Material
- bcsB(A.xylinum) and bcsA,B,C(E.coli)
- you can see other materials at you can see other materials at protocol7
- Procedure
- see protocol7
Preparation:Make preculture
- Member
- naoto and NEX
- Material
- LB+Cam Broth
- a colony of E.coli having bcsB(A.xylinum)
- you can see other materials at you can see other materials at protocol2
- Procedure
- see protocol2
