Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/17

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/16=
 
=2010/10/17=
=2010/10/17=
===1:PCR===
===1:PCR===
 +
<Member><br />
 +
nito
 +
 +
<Sample>(temperature in annealing:℃)<br />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]<br />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
 +
===2:DNA Ligation===
===2:DNA Ligation===
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＜Member＞<br />
 +
Takahiro
 +
 +
＜Sample＞<br />
 +
*bcsB-pvC 
 +
*bcsC-pvC
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*bcsB-vector
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*bcsC-vector
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]-vector
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]-vector
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]-[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]-[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]-[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
 +
(after digestion)
 +
 +
＜Protocol＞<br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
 +
===3:Electrophoresis===
===3:Electrophoresis===
<Protocol><br />
<Protocol><br />
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<Result><br />
<Result><br />
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[[Image:]]
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[[Image:Matsuura_2010-10-17_20hr_08min.JPG]]
===6:PCR===
===6:PCR===
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===8:DNA Digestion===
===8:DNA Digestion===
===9:Electrophoresis===
===9:Electrophoresis===
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<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]<br />
 +
 +
<Result><br />
 +
[[Image:Matsuura_2010-10-17_22hr_08min.JPG‎ ]]
 +
===10:Electrophoresis===
===10:Electrophoresis===
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]<br />
 +
 +
<Result><br />
 +
[[Image:Matsuura_2010-10-18_01hr_41min.JPG‎]]
 +
===11:DNA extraction===
===11:DNA extraction===
===12:DNA extraction from gels===
===12:DNA extraction from gels===
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]<br />
 +
 +
<Result><br />
 +
[[Image:Matsuura_2010-10-18_03hr_10min.JPG‎]]
 +
===13:DNA extraction===
===13:DNA extraction===
===14:PCR===
===14:PCR===

Latest revision as of 14:07, 27 October 2010


E.coli Pattern Formation Project Notebook



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September 2010
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Contents

2010/10/17

1:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 1

2:DNA Ligation

＜Member＞
Takahiro

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 3

3:Electrophoresis

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-17 19hr 41min.JPG

Matsuura 2010-10-17 19hr 54min.JPG

4:Electrophoresis

<Protocol> See Protocol 8

<Result>
Matsuura 2010-10-17 21hr 09min.JPG

Matsuura 2010-10-17 21hr 43min.JPG

Matsuura 2010-10-17 22hr 07min.JPG

5:DNA extraction from gels

<Protocol>
See Protocol 4

<Result>
Matsuura 2010-10-17 20hr 08min.JPG

6:PCR

7:Transformation

8:DNA Digestion

9:Electrophoresis

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-17 22hr 08min.JPG

10:Electrophoresis

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-18 01hr 41min.JPG

11:DNA extraction

12:DNA extraction from gels

<Protocol>
See Protocol 4

<Result>
Matsuura 2010-10-18 03hr 10min.JPG

13:DNA extraction

14:PCR

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/17"