E.coli Pattern Formation Project Notebook

August 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2010 SUN MON TUE WED THU FRI SAT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Contents 1 2010/10/17 1.1 1:PCR 1.2 2:DNA Ligation 1.3 3:Electrophoresis 1.4 4:Electrophoresis 1.5 5:DNA extraction from gels 1.6 6:PCR 1.7 7:Transformation 1.8 8:DNA Digestion 1.9 9:Electrophoresis 1.10 10:Electrophoresis 1.11 11:DNA extraction 1.12 12:DNA extraction from gels 1.13 13:DNA extraction 1.14 14:PCR

2010/10/17

1:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

• 1-2-7
  
• 7-8-10

<Protocol>
See Protocol 1

2:DNA Ligation

＜Member＞
Takahiro

＜Sample＞

• bcsB-pvC
• bcsC-pvC
• bcsB-vector
• bcsC-vector
• 1-2-7-vector
• 10-2-7-vector
• 10-2-3
• 4-5
• 1-2-3

(after digestion)

＜Protocol＞
See Protocol 3

3:Electrophoresis

<Protocol>
See Protocol 8

<Result>

4:Electrophoresis

<Protocol> See Protocol 8

<Result>

5:DNA extraction from gels

<Protocol>
See Protocol 4

<Result>

6:PCR

7:Transformation

8:DNA Digestion

9:Electrophoresis

<Protocol>
See Protocol 8

<Result>

10:Electrophoresis

<Protocol>
See Protocol 8

<Result>

11:DNA extraction

12:DNA extraction from gels

<Protocol>
See Protocol 4

<Result>

13:DNA extraction

14:PCR