"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16
From 2010.igem.org
(Difference between revisions)
(→Experiment:PCR) |
|||
| (One intermediate revision not shown) | |||
| Line 11: | Line 11: | ||
:'''Materials,Procedure''' | :'''Materials,Procedure''' | ||
| - | :Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis"> | + | :Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html> |
:*95℃3min | :*95℃3min | ||
:*96℃1min | :*96℃1min | ||
| Line 19: | Line 19: | ||
:（96℃～72℃/30cycle） | :（96℃～72℃/30cycle） | ||
| - | ===Experiment:Agarose | + | ===Experiment:Agarose gel Electrophoresis=== |
:'''Member''' | :'''Member''' | ||
| Line 28: | Line 28: | ||
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html> | :Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html> | ||
| - | :PCR production (pSB1C3) | + | :*PCR production (pSB1C3) |
===Preparation:Make Agarose Gel Electrophoresis=== | ===Preparation:Make Agarose Gel Electrophoresis=== | ||
Latest revision as of 15:36, 27 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/09/16 Thuesday (Bambi75)
Experiment:PCR
- Member
- naoto and bambi75
- Materials,Procedure
- Same protocol4
- 95℃3min
- 96℃1min
- 51℃1min
- 72℃3min
- 10℃∞
- （96℃～72℃/30cycle）
Experiment:Agarose gel Electrophoresis
- Member
- Same above
- Materials,Procedure
- Same protocol4
- PCR production (pSB1C3)
Preparation:Make Agarose Gel Electrophoresis
- Member
- Same above
- Materials and Procedure
- Same protocol4
Preparation:Medium making
- Member
- Same above
- Materials and Procedure
- Same protocol1
Experiment:Ligation
- Member
- Same above
- Materials and Procedure
- follow protocol8
- bcsA,B,C and D
- pSB1C3
