Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

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(Experiment:PCR)
 
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:'''Materials,Procedure'''
:'''Materials,Procedure'''
-
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol3</a></html>
+
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
:*95℃3min
:*95℃3min
:*96℃1min
:*96℃1min
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:(96℃~72℃/30cycle)
:(96℃~72℃/30cycle)
-
===Experiment:Agarose Gel Electrophoresis===
+
===Experiment:Agarose gel Electrophoresis===
:'''Member'''
:'''Member'''
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:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
:PCR production (pSB1C3)
+
:*PCR production (pSB1C3)
===Preparation:Make Agarose Gel Electrophoresis===
===Preparation:Make Agarose Gel Electrophoresis===

Latest revision as of 15:36, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member
naoto and bambi75
Materials,Procedure
Same protocol4
  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞
(96℃~72℃/30cycle)

Experiment:Agarose gel Electrophoresis

Member
Same above
Materials,Procedure
Same protocol4
  • PCR production (pSB1C3)

Preparation:Make Agarose Gel Electrophoresis

Member
Same above
Materials and Procedure
Same protocol4

Preparation:Medium making

Member
Same above
Materials and Procedure
Same protocol1

Experiment:Ligation

Member
Same above
Materials and Procedure
follow protocol8
  • bcsA,B,C and D
  • pSB1C3