"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13
From 2010.igem.org
(Difference between revisions)
(→Experiment:Restrict enzyme digestion) |
|||
| Line 11: | Line 11: | ||
:'''Materials''' | :'''Materials''' | ||
| - | :* | + | :*bcsA,B,C,D(each):50μl |
:*10×M buffer:15μl | :*10×M buffer:15μl | ||
:*BSA:15μl | :*BSA:15μl | ||
Latest revision as of 21:28, 26 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/08/13 Friday (bambi75)
Experiment:Restrict enzyme digestion
- Member
- NEX , bambi75 and watachin
- Materials
- bcsA,B,C,D(each):50μl
- 10×M buffer:15μl
- BSA:15μl
- XbaI:3μl
- SpeI:3μl
- Procedure
- mix materials : M buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
- incubation(37℃).
