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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/21
From 2010.igem.org
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__NOTOC__ | __NOTOC__ | ||
==2010/10/21 Thursday (Naoto)== | ==2010/10/21 Thursday (Naoto)== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and watachin | + | :naoto and watachin |
===Experiment:PCR=== | ===Experiment:PCR=== | ||
| - | '''material''' | + | :'''material''' |
| - | *templete DNA (from A.xylinum and pSB1C3) | + | :*templete DNA (from A.xylinum and pSB1C3) |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | '''material''' | + | :'''material''' |
| - | *PCR production(bcsA and bcsB) | + | :*PCR production(bcsA and bcsB) |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
===Experiment:DNA purification with silica gel=== | ===Experiment:DNA purification with silica gel=== | ||
| - | '''material''' | + | :'''material''' |
| - | *PCR production(bcsD and pSB1C3) | + | :*PCR production(bcsD and pSB1C3) |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | '''material''' | + | :'''material''' |
| - | *PCR production (bcsA,bcsB,bcsC and bcsD) | + | :*PCR production (bcsA,bcsB,bcsC and bcsD) |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> |
===Experiment:Digestion=== | ===Experiment:Digestion=== | ||
| - | '''material''' | + | :'''material''' |
| - | *bcsC | + | :*bcsC |
| - | *bcsD | + | :*bcsD |
| - | *XbaI | + | :*XbaI |
| - | *SpeI | + | :*SpeI |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> |
===Experiment:DNA purification with silica gel=== | ===Experiment:DNA purification with silica gel=== | ||
| - | '''material''' | + | :'''material''' |
| - | *PCR production(bcsC and bcsD) | + | :*PCR production(bcsC and bcsD) |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
| - | '''material''' | + | :'''material''' |
| - | *bcsC and bcsD | + | :*bcsC and bcsD |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> |
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
| - | '''material''' | + | :'''material''' |
| - | *Competent cell(Ecos JM109) | + | :*Competent cell(Ecos JM109) |
| - | *bcsD in pSB1C3 | + | :*bcsD in pSB1C3 |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
<br/> | <br/> | ||
Latest revision as of 18:04, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/21 Thursday (Naoto)
- member
- naoto and watachin
Experiment:PCR
- material
- templete DNA (from A.xylinum and pSB1C3)
- you can see other materials at protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- PCR production(bcsA and bcsB)
- you can see materials at protocol4
- procedure
- refer to protocol4
Experiment:DNA purification with silica gel
- material
- PCR production(bcsD and pSB1C3)
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Electrophoresis
- material
- PCR production (bcsA,bcsB,bcsC and bcsD)
- you can see other materials at protocol4
- procedure
- see protocol4
Experiment:Digestion
- material
- bcsC
- bcsD
- XbaI
- SpeI
- you can see other materials at protocol6
- procedure
- see protocol6
Experiment:DNA purification with silica gel
- material
- PCR production(bcsC and bcsD)
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Ligation
- material
- bcsC and bcsD
- you can see materials at protocol7
- procedure
- refer to protocol7
Experiment:Transformation
- material
- Competent cell(Ecos JM109)
- bcsD in pSB1C3
- you can see materials at protocol8
- procedure
- see protocol8
