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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14
From 2010.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
==2010/10/14 Thursday (Naoto)== | ==2010/10/14 Thursday (Naoto)== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto,maki and watachin | + | :naoto,maki and watachin |
===Preparation:Measure DNA concentration=== | ===Preparation:Measure DNA concentration=== | ||
| - | '''Material''' | + | :'''Material''' |
| - | *bcsA | + | :*bcsA |
| - | *bcsB | + | :*bcsB |
| - | *bcsC | + | :*bcsC |
| - | *bcsD | + | :*bcsD |
| - | *pSB1C3 | + | :*pSB1C3 |
| - | *TE buffer | + | :*TE buffer |
| - | '''Procedure''' | + | :'''Procedure''' |
| - | #mix 4μl of DNA and 4μl of TE buffer | + | :#mix 4μl of DNA and 4μl of TE buffer |
| - | #set mixture in absorption spectrometer | + | :#set mixture in absorption spectrometer |
| - | #measure DNA concentrations | + | :#measure DNA concentrations |
===Experiment:PCR=== | ===Experiment:PCR=== | ||
| - | '''material''' | + | :'''material''' |
| - | *templete DNA (from A.xylinum and pSB1C3) | + | :*templete DNA (from A.xylinum and pSB1C3) |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | '''material''' | + | :'''material''' |
| - | *bcsA | + | :*bcsA |
| - | *bcsB | + | :*bcsB |
| - | *bcsC | + | :*bcsC |
| - | *bcsD | + | :*bcsD |
| - | *pSB1C3 | + | :*pSB1C3 |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
<br/> | <br/> | ||
Latest revision as of 17:57, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/14 Thursday (Naoto)
- member
- naoto,maki and watachin
Preparation:Measure DNA concentration
- Material
- bcsA
- bcsB
- bcsC
- bcsD
- pSB1C3
- TE buffer
- Procedure
- mix 4μl of DNA and 4μl of TE buffer
- set mixture in absorption spectrometer
- measure DNA concentrations
Experiment:PCR
- material
- templete DNA (from A.xylinum and pSB1C3)
- you can see other materials at protocol3
- procedure
- see protocol3
Experiment:Electrophoresis
- material
- bcsA
- bcsB
- bcsC
- bcsD
- pSB1C3
- you can see materials at protocol4
- procedure
- refer to protocol4
