Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25

From 2010.igem.org

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==2010/09/10 Saturday(NEX)==
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/09/25 Saturday(NEX)==
===Experiment:PCR===
===Experiment:PCR===
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'''Member'''<br/>
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:'''Member'''<br/>
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easily
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:easily
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'''Material'''<br/>
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:'''Material'''<br/>
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*sense primers of pSB1C3
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:*templete DNA:pSB1C3
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*antisense primers of pSB1C3
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:*sense primers of pSB1C3
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:*antisense primers of pSB1C3
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'''Procedure'''<br/>
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Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html>
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:'''Procedure'''<br/>
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:Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html>
===Experiment:Ligatiom===
===Experiment:Ligatiom===
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'''Member'''<br/>
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:'''Member'''<br/>
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easily   
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:easily   
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'''Material'''<br/>
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:'''Material'''<br/>
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*bcsA from E.coli 20μl
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:*bcsA from E.coli 20μl
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*bcsB from E.coli 20μl
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:*bcsB from E.coli 20μl
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*2×ligation mix  40μl
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:*2×ligation mix  40μl
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'''Procedure'''<br/>
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Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
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 +
:'''Procedure'''<br/>
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:Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
===Experiment:Agarose gel electrophoresis===
===Experiment:Agarose gel electrophoresis===
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'''Member'''<br />
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:'''Member'''<br />
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easily
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:easily
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'''Material'''
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:'''Material'''
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*Today's PCR productions
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:*Today's PCR productions
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*Today's ligation productions
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:*Today's ligation productions
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*Agarose gel
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:*Agarose gel
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'''Procedure'''<br/>
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:'''Procedure'''<br/>
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Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html>
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:Refer to <html><a href="Agarose gel electrophoresis">protocol4</a></html>
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'''Result'''<br/>
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:'''Result'''<br/>
[[Image:100925.jpg]]<br/>
[[Image:100925.jpg]]<br/>
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pSB1C3 were appeared
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:pSB1C3 were appeared
===Experiment:DNA purification with silica gel===
===Experiment:DNA purification with silica gel===
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'''Member'''<br />
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:'''Member'''<br />
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easily
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:easily
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'''Materials'''<br />
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:'''Materials'''<br />
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*pSB1C3
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:*pSB1C3
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'''Procedure'''<br />
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:'''Procedure'''<br />
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See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol5-2:DNA_Purification_with_silica_gel">protocol5-2</a></html>
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:See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel">protocol6</a></html>
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===Experiment:Ligatiom===
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:'''Member'''<br/>
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:easily 
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:'''Material'''<br/>
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:*pSB1C3 3μl
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:*bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
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:*2×ligation mix  6μl
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===Experiment:Ligatiom===
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:'''Procedure'''<br/>
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'''Member'''<br/>
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:Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
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easily 
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-
 
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'''Material'''<br/>
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*pSB1C3 3μl
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*bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
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*2×ligation mix  6μl
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'''Procedure'''<br/>
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<br/>
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Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html>
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Latest revision as of 14:57, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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31

2010/09/25 Saturday(NEX)

Experiment:PCR

Member
easily
Material
  • templete DNA:pSB1C3
  • sense primers of pSB1C3
  • antisense primers of pSB1C3
Procedure
Refer to protocol3

Experiment:Ligatiom

Member
easily
Material
  • bcsA from E.coli 20μl
  • bcsB from E.coli 20μl
  • 2×ligation mix 40μl
Procedure
Follow to protocol7

Experiment:Agarose gel electrophoresis

Member
easily
Material
  • Today's PCR productions
  • Today's ligation productions
  • Agarose gel
Procedure
Refer to protocol4
Result

100925.jpg

pSB1C3 were appeared

Experiment:DNA purification with silica gel

Member
easily
Materials
  • pSB1C3
Procedure
See protocol6

Experiment:Ligatiom

Member
easily
Material
  • pSB1C3 3μl
  • bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
  • 2×ligation mix 6μl
Procedure
Follow to protocol7

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