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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/10
From 2010.igem.org
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__NOTOC__ | __NOTOC__ | ||
==2010/10/10 Sunday (Naoto)== | ==2010/10/10 Sunday (Naoto)== | ||
| - | '''member''' | + | :'''member''' |
| - | watachin | + | :watachin |
===Preparation:Measure DNA concentration=== | ===Preparation:Measure DNA concentration=== | ||
| - | '''material''' | + | :'''material''' |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
| - | '''material''' | + | :'''material''' |
| - | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
| - | '''material''' | + | :'''material''' |
| - | *Competent Cell (Novablue) | + | :*Competent Cell (Novablue) |
| - | *bcsA in pSB1C3 | + | :*bcsA in pSB1C3 |
| - | *bcsB in pSB1C3 | + | :*bcsB in pSB1C3 |
| - | *bcsC in pSB1C3 | + | :*bcsC in pSB1C3 |
| - | *bcsD in pSB1C3 | + | :*bcsD in pSB1C3 |
| - | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html> |
| - | '''procedure''' | + | :'''procedure''' |
| - | + | ||
| - | + | ||
| + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html> | ||
<br/> | <br/> | ||
Latest revision as of 18:10, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/10 Sunday (Naoto)
- member
- watachin
Preparation:Measure DNA concentration
- material
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Ligation
- material
- you can see materials at protocol8
- procedure
- refer to protocol8
Experiment:Transformation
- material
- Competent Cell (Novablue)
- bcsA in pSB1C3
- bcsB in pSB1C3
- bcsC in pSB1C3
- bcsD in pSB1C3
- you can see other materials at protocol9
- procedure
- refer to protocol9
