Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/24

From 2010.igem.org

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==2010/9/24 Friday (watachin)==
==2010/9/24 Friday (watachin)==
===Experiment:PCR===
===Experiment:PCR===
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'''Member'''<br />
+
:'''Member'''<br />
-
easily
+
:easily
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
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Direct PCR of bcs genes
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:templete DNA (bcsA,B,C,D)
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Colony PCR===
===Experiment:Colony PCR===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
Colony PCR of bcs genes
+
:E.coli induced bcsA,B,C and D
-
 
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol</a></html>
+
===Experiment:Ligation===
===Experiment:Ligation===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily
+
:easily
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">the protocol(Ligation)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol3</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
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'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
+
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily and watachin
+
:easily and watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:'''Result'''
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+
-
 
+
-
'''Result'''
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[[Image:2010-09-24-01.jpg]]
[[Image:2010-09-24-01.jpg]]
===Experiment:Transformation===
===Experiment:Transformation===
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'''Member'''<br />
+
:'''Member'''<br />
-
easily and watachin
+
:easily and watachin
-
 
+
-
'''Materials and Procedure'''
+
 +
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>

Latest revision as of 14:33, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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19 20 21 22 23 24 25
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October 2010
SUNMONTUEWEDTHUFRISAT
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24 25 26 27 28 29 30
31

2010/9/24 Friday (watachin)

Experiment:PCR

Member
easily
Materials and Procedure
templete DNA (bcsA,B,C,D)
follow protocol3

Experiment:Colony PCR

Member
watachin
Materials and Procedure
E.coli induced bcsA,B,C and D
follow protocol3

Experiment:Ligation

Member
easily
Materials and Procedure
follow protocol3

Experiment:Digestion

Member
watachin
Materials and Procedure
follow protocol7

Experiment:Electrophoresis

Member
easily and watachin
Materials and Procedure
follow protocol4
Result

2010-09-24-01.jpg

Experiment:Transformation

Member
easily and watachin
Materials and Procedure
follow protocol9
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