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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23
From 2010.igem.org
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==2010/9/23 Thursday (watachin)== | ==2010/9/23 Thursday (watachin)== | ||
===Experiment:PCR=== | ===Experiment:PCR=== | ||
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | naoto | + | :naoto |
| - | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
| - | + | :E.coli introduced bcsA,B,C,D | |
| - | + | ||
| - | + | ||
| + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | ||
===Experiment:Plating=== | ===Experiment:Plating=== | ||
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | watachin | + | :watachin |
| - | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
| - | plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth | + | :plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth |
| + | ===Experiment:Colony PCR=== | ||
| + | :'''Member'''<br /> | ||
| + | :naoto | ||
| - | + | :'''Materials and Procedure''' | |
| - | ''' | + | |
| - | + | ||
| - | + | :Colony PCR production of bcs genes | |
| - | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | |
| - | + | ||
| - | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR"> | + | |
===Experiment:Insert check Electrophoresis=== | ===Experiment:Insert check Electrophoresis=== | ||
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | watachin | + | :watachin |
| - | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
| - | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html> |
| - | '''Result''' | + | :'''Result''' |
[[Image:2010-09-23-01.jpg]] | [[Image:2010-09-23-01.jpg]] | ||
| Line 46: | Line 44: | ||
===Experiment:Electrophoresis of bcs genes=== | ===Experiment:Electrophoresis of bcs genes=== | ||
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
| - | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html> |
| - | ''' | + | ===Experiment:DNA Extraction from agarose gel=== |
| + | :'''Member'''<br /> | ||
| + | :naoto and watachin | ||
| + | :'''Materials and Procedure''' | ||
| - | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> | |
| - | + | ||
| - | + | ||
| - | ''' | + | ===Experiment:Electrophoresis=== |
| + | :'''Member'''<br /> | ||
| + | :naoto and watachin | ||
| + | :'''Materials and Procedure''' | ||
| + | :*Colony PCR production | ||
| + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html> | ||
| - | + | :'''Result''' | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | '''Result''' | + | |
[[Image:2010-09-23-03.jpg]] | [[Image:2010-09-23-03.jpg]] | ||
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | watachin | + | :watachin |
| - | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
| - | + | ||
| - | + | ||
| - | + | ||
| + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html> | ||
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | naoto and watachin | + | :naoto and watachin |
| - | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
| - | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html> |
| - | '''Result''' | + | :'''Result''' |
[[Image:2010-09-23-04.jpg]] | [[Image:2010-09-23-04.jpg]] | ||
| - | ===Experiment:DNA | + | ===Experiment:DNA Extraction from agarose gel=== |
| - | '''Member'''<br /> | + | :'''Member'''<br /> |
| - | naoto and watachin | + | :naoto and watachin |
| - | + | ||
| - | + | ||
| + | :'''Materials and Procedure''' | ||
| - | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> |
Latest revision as of 14:27, 27 October 2010
E.coli Fiber Project Notebook
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2010/9/23 Thursday (watachin)
Experiment:PCR
- Member
- naoto
- Materials and Procedure
- E.coli introduced bcsA,B,C,D
- follow protocol3
Experiment:Plating
- Member
- watachin
- Materials and Procedure
- plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Colony PCR
- Member
- naoto
- Materials and Procedure
- Colony PCR production of bcs genes
- follow protocol3
Experiment:Insert check Electrophoresis
- Member
- watachin
- Materials and Procedure
- follow protocol5
- Result
Experiment:Electrophoresis of bcs genes
- Member
- naoto and watachin
- Materials and Procedure
Experiment:DNA Extraction from agarose gel
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol5
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- Colony PCR production
- follow protocol4
- Result
Experiment:Transformation
- Member
- watachin
- Materials and Procedure
- follow protocol9
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol4
- Result
Experiment:DNA Extraction from agarose gel
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol5
