Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

From 2010.igem.org

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===Experiment:Agarose Gels Electrophoresis===
===Experiment:Agarose Gels Electrophoresis===
-
'''member'''
+
:'''member'''
-
NEX , bambi75 and watachin
+
:NEX , bambi75 and watachin
-
'''Materials,Procedure'''
+
:'''Materials,Procedure'''
-
Same protocol on August 16th.
+
:Same protocol on August 16th.
-
PCR Product of insert are bcsA and bcsC.
+
:PCR Product of insert are bcsA and bcsC.
===Experiment:PCR===
===Experiment:PCR===
-
'''member'''
+
:'''Member'''
-
NEX , bambi75 and watachin
+
:NEX , bambi75 and watachin
-
'''material'''
+
:'''Material'''
-
*sterilized water 213μl
+
:*sterilized water 213μl
-
*Ex taq buffer 30μl
+
:*Ex taq buffer 30μl
-
*dNTP 24μl
+
:*dNTP 24μl
-
*Ex taq 3μl
+
:*Ex taq 3μl
-
*K12bcsA sense 15μl
+
:*bcsA sense 15μl
-
*K12bcsA antisense 15μl
+
:*bcsA antisense 15μl
-
'''Procedure'''
+
:'''Procedure'''
-
 
+
-
Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
 +
:Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
===Experiment:Restriction enzyme digestion===
===Experiment:Restriction enzyme digestion===
-
'''member'''
+
:'''Member'''
-
Same above
+
:Same above
-
'''Materials'''
+
:'''Materials'''
-
*PCR production
+
:*PCR production
-
*m buffer 30μl
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:*M buffer 30μl
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*BSA 30μl
+
:*BSA 30μl
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*Xba1 6μl
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:*Xba1 6μl
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*Spe1 6μl
+
:*Spe1 6μl
-
 
+
-
'''Procedure'''
+
-
 
+
-
Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol4</a></html>
+
 +
:'''Procedure'''
 +
:Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol7</a></html>
===Experiment:Transformation===
===Experiment:Transformation===
-
'''member'''
+
:'''Member'''
-
 
+
-
Same above
+
-
 
+
-
'''Materials , Procedure'''
+
-
 
+
-
Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html>
+
 +
:Same above
 +
:'''Materials , Procedure'''
 +
:Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html>
<br/>
<br/>

Latest revision as of 21:45, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member
NEX , bambi75 and watachin
Materials,Procedure
Same protocol on August 16th.
PCR Product of insert are bcsA and bcsC.

Experiment:PCR

Member
NEX , bambi75 and watachin
Material
  • sterilized water 213μl
  • Ex taq buffer 30μl
  • dNTP 24μl
  • Ex taq 3μl
  • bcsA sense 15μl
  • bcsA antisense 15μl
Procedure
Please see protocol3

Experiment:Restriction enzyme digestion

Member
Same above
Materials
  • PCR production
  • M buffer 30μl
  • BSA 30μl
  • Xba1 6μl
  • Spe1 6μl
Procedure
Please see protocol7

Experiment:Transformation

Member
Same above
Materials , Procedure
Please refer to protocol9