Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

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===Experiment:PCR===
===Experiment:PCR===
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'''member'''
+
:'''Member'''
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naoto and bambi75
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:naoto and bambi75
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'''materials,Procedure'''
+
:'''Materials,Procedure'''
-
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
*95℃3min
+
:*95℃3min
-
*96℃1min
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:*96℃1min
-
*51℃1min
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:*51℃1min
-
*72℃3min
+
:*72℃3min
-
*10℃∞
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:*10℃∞
-
（96℃～72℃/30cycle）
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:（96℃～72℃/30cycle）
-
===Experiment:Agarose Gels Electro-phoresis===
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===Experiment:Agarose gel Electrophoresis===
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'''member'''
+
:'''Member'''
-
Same above
+
:Same above
-
'''Materials,Procedure'''
+
:'''Materials,Procedure'''
-
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
-
PCR Product of insert is pBS1C3
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:*PCR production (pSB1C3)
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===Experiment:Experiment:Make Agarose Gels Electro-phoresis===
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===Preparation:Make Agarose Gel Electrophoresis===
-
'''Member'''
+
:'''Member'''
-
Same above  
+
:Same above  
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
+
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
===Preparation:Medium making===
===Preparation:Medium making===
-
'''member'''
+
:'''Member'''
-
Same above
+
:Same above
-
'''materials and procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
+
 +
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol1</a></html>
===Experiment:Ligation===
===Experiment:Ligation===
-
'''member'''
+
:'''Member'''
-
 
+
-
Same above
+
-
 
+
-
'''materials and procedure'''
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
+
-
*bcsA,B and C(E.coli).
+
-
*bcsA,B,C and D(A.xylium)
+
-
*pSB1C3
+
-
 
+
 +
:Same above
 +
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol8</a></html>
 +
:*bcsA,B,C and D
 +
:*pSB1C3
<br/>
<br/>

Latest revision as of 15:36, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
SUNMONTUEWEDTHUFRISAT
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October 2010
SUNMONTUEWEDTHUFRISAT
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31


2010/09/16 Thuesday (Bambi75)

Experiment:PCR

Member
naoto and bambi75
Materials,Procedure
Same protocol4
  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞
（96℃～72℃/30cycle）

Experiment:Agarose gel Electrophoresis

Member
Same above
Materials,Procedure
Same protocol4
  • PCR production (pSB1C3)

Preparation:Make Agarose Gel Electrophoresis

Member
Same above
Materials and Procedure
Same protocol4

Preparation:Medium making

Member
Same above
Materials and Procedure
Same protocol1

Experiment:Ligation

Member
Same above
Materials and Procedure
follow protocol8
  • bcsA,B,C and D
  • pSB1C3

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/16"