Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/07

From 2010.igem.org

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==2010/09/07 Tuesday (Bambi75)==
==2010/09/07 Tuesday (Bambi75)==
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===Experiment:Agarose Gels Electro-phoresis===
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===Experiment:Agarose gel Electrophoresis===
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'''member'''
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:'''member'''
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bambi75 and watachin
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:bambi75 and watachin
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'''Materials'''
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:'''Material'''
 +
:*PCR production
 +
:*10×Loading Buffer
 +
:*DNA maker
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*10×Loading Buffer 30μl
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:'''Procedure'''
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*DNA maker 15μl
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'''Procedure'''
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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+
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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===Experiment:Extraction of DNA from　agarose gel===
===Experiment:Extraction of DNA from　agarose gel===
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'''member'''
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:'''member'''
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Same above
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:Same above
-
 
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'''Materials'''
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-
 
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*QB Buffer 1800μl
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*PE Buffer 4200μl
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*EB 300μl
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(sum volume)
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'''Procedure'''
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-
 
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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
+
 +
:'''Material'''
 +
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
:'''Procedure'''
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
<br/>
<br/>

Latest revision as of 06:37, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/07 Tuesday (Bambi75)

Experiment:Agarose gel Electrophoresis

member
bambi75 and watachin
Material
  • PCR production
  • 10×Loading Buffer
  • DNA maker
Procedure
see protocol4

Experiment:Extraction of DNA from　agarose gel

member
Same above
Material
you can see materials at protocol5
Procedure
refer to protocol5

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/07"