Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/06

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/10/06 Wednesday (Naoto)==
==2010/10/06 Wednesday (Naoto)==
===Experiment:Electrophoresis of insert check PCR===
===Experiment:Electrophoresis of insert check PCR===
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'''member'''
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:'''member'''
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naoto
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:naoto
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'''material'''
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:'''material'''
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*insert check PCR production(PCR at 10/05)  
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:*insert check PCR production(PCR at 10/05)  
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If you want to know other materials,see protocol4
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:If you want to know other materials,see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
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'''procedure'''
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:'''procedure'''
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see protocol4
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:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
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'''result'''
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:'''result'''
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All of bands seemed to be vector self ligation
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:All of bands seemed to be vector self ligation
===Experiment:PCR of  T7promoter~bcsABCD(''A.xylinum''),T7promoter~bcsEFG(''E.coli''),RBS~T7polymerase===
===Experiment:PCR of  T7promoter~bcsABCD(''A.xylinum''),T7promoter~bcsEFG(''E.coli''),RBS~T7polymerase===
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'''member'''
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:'''member'''
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naoto
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:naoto
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'''material'''
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:'''material'''
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*A colony of ''A.xylinum''
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:*A colony of ''A.xylinum''
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*bcsEFG(''E.coli'')
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:*bcsEFG(''E.coli'')
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*T7 polymerase(BBa_K145001)
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:*T7 polymerase(BBa_K145001)
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If you want to know other materials,see protocol3
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:If you want to know other materials,see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
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'''procedure'''
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:'''procedure'''
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see protocol3
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:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
===Experiment:Measure DNA density===
===Experiment:Measure DNA density===
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'''member'''
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:'''member'''
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:naoto
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:'''material'''
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:*absorption spectrometer
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:*bcsA,B,C,D(''A.xylinum'')
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:*bcsA,B,C,EFG(''E.coli'')
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:*pSB1C3
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:*TE buffer
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:'''procedure'''
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naoto
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:*mix 2µl DNA solution with 2μl TE buffer
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:*apply DNA solution and TE buffer mixture to absorption spectrometer
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:*measure DNA density
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'''material'''
 
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*absorption spectrometer
 
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*bcsA,B,C,D(''A.xylinum'')
 
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*bcsA,B,C,EFG(''E.coli'')
 
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*pSB1C3
 
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*TE buffer
 
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'''procedure'''
 
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*mix 2µl DNA solution with 2μl TE buffer
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<br />
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*apply DNA solution and TE buffer mixture to absorption spectrometer
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*measure DNA density
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Latest revision as of 18:13, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/10/06 Wednesday (Naoto)

Experiment:Electrophoresis of insert check PCR

member
naoto
material
  • insert check PCR production(PCR at 10/05)
If you want to know other materials,see protocol4
procedure
see protocol4
result
All of bands seemed to be vector self ligation

Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase

member
naoto
material
  • A colony of A.xylinum
  • bcsEFG(E.coli)
  • T7 polymerase(BBa_K145001)
If you want to know other materials,see protocol3
procedure
see protocol3

Experiment:Measure DNA density

member
naoto
material
  • absorption spectrometer
  • bcsA,B,C,D(A.xylinum)
  • bcsA,B,C,EFG(E.coli)
  • pSB1C3
  • TE buffer
procedure
  • mix 2µl DNA solution with 2μl TE buffer
  • apply DNA solution and TE buffer mixture to absorption spectrometer
  • measure DNA density




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