Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/07

From 2010.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
 +
==2010/09/07 Tuesday (Bambi75)==
 +
===Experiment:Agarose gel Electrophoresis===
-
===Agarose Gels Electro-phoresis===
+
:'''member'''
-
'''member'''
+
:bambi75 and watachin
-
bambi75 and watachin
+
-
'''Materials'''
+
:'''Material'''
-
*10×Loading Buffer 30μl
+
:*PCR production
-
*DNA maker 15μl
+
:*10×Loading Buffer  
 +
:*DNA maker  
-
'''Procedure'''
+
:'''Procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
+
-
===collect DNA===
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''member'''
+
===Experiment:Extraction of DNA from agarose gel===
-
Same above
+
-
'''Materials'''
+
:'''member'''
-
*QB Buffer 1800μl
+
:Same above
-
*PE Buffer 4200μl
+
-
*EB 300μl
+
-
'''Procedure'''
+
:'''Material'''
-
<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
+
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
 
 +
:'''Procedure'''
 +
 
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
 
 +
<br/>

Latest revision as of 06:37, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/07 Tuesday (Bambi75)

Experiment:Agarose gel Electrophoresis

member
bambi75 and watachin
Material
  • PCR production
  • 10×Loading Buffer
  • DNA maker
Procedure
see protocol4

Experiment:Extraction of DNA from agarose gel

member
Same above
Material
you can see materials at protocol5
Procedure
refer to protocol5