Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/07

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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==2010/09/07 Tuesday (Bambi75)==
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===Experiment:Agarose gel Electrophoresis===
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===Agarose Gels Electro-phoresis===
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:'''member'''
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'''member'''
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:bambi75 and watachin
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bambi75 and watachin
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'''Materials'''
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:'''Material'''
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*10×Loading Buffer 30μl
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:*PCR production
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*DNA maker 15μl
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:*10×Loading Buffer  
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:*DNA maker  
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'''Procedure'''
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:'''Procedure'''
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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===collect DNA===
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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'''member'''
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===Experiment:Extraction of DNA from　agarose gel===
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Same above
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'''Materials'''
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:'''member'''
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*QB Buffer 1800μl
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:Same above
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*PE Buffer 4200μl
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*EB 300μl
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'''Procedure'''
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:'''Material'''
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<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
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:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
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:'''Procedure'''
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:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
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<br/>

Latest revision as of 06:37, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/07 Tuesday (Bambi75)

Experiment:Agarose gel Electrophoresis

member
bambi75 and watachin
Material
  • PCR production
  • 10×Loading Buffer
  • DNA maker
Procedure
see protocol4

Experiment:Extraction of DNA from　agarose gel

member
Same above
Material
you can see materials at protocol5
Procedure
refer to protocol5

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/07"