Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

From 2010.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
 
+
__NOTOC__
==2010/08/24 Tuesday(bambi75)==
==2010/08/24 Tuesday(bambi75)==
-
 
===Experiment:Agarose Gels Electrophoresis===
===Experiment:Agarose Gels Electrophoresis===
-
'''member'''
+
:'''member'''
-
NEX , bambi75 and watachin
+
-
'''Materials,Procedure'''
+
:NEX , bambi75 and watachin
-
Same protocol on August 16th.
+
 
-
PCR Product of insert are bcsA and bcsC.
+
:'''Materials,Procedure'''
 +
 
 +
:Same protocol on August 16th.
 +
:PCR Product of insert are bcsA and bcsC.
===Experiment:PCR===
===Experiment:PCR===
-
'''member'''
+
:'''Member'''
-
NEX , bambi75 and watachin
+
:NEX , bambi75 and watachin
-
'''material'''
+
:'''Material'''
-
*sterilized water 213μl
+
:*sterilized water 213μl
-
*Ex taq buffer 30μl
+
:*Ex taq buffer 30μl
-
*dNTP 24μl
+
:*dNTP 24μl
-
*Ex taq 3μl
+
:*Ex taq 3μl
-
*K12bcsA sense 15μl
+
:*bcsA sense 15μl
-
*K12bcsA antisense 15μl
+
:*bcsA antisense 15μl
-
'''Procedure'''
+
:'''Procedure'''
-
Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
 +
:Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
===Experiment:Restriction enzyme digestion===
===Experiment:Restriction enzyme digestion===
-
'''member'''
+
:'''Member'''
-
 
+
-
Same above
+
-
'''Materials'''
+
:Same above
-
*PCR production
+
:'''Materials'''
-
*m buffer 30μl
+
-
*BSA 30μl
+
-
*Xba1 6μl
+
-
*Spe1 6μl
+
-
'''Procedure'''
+
:*PCR production
 +
:*M buffer 30μl
 +
:*BSA 30μl
 +
:*Xba1 6μl
 +
:*Spe1 6μl
-
Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol4</a></html>
+
:'''Procedure'''
 +
:Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol7</a></html>
 +
===Experiment:Transformation===
-
==Experiment:Transformation==
+
:'''Member'''
-
'''member'''
+
:Same above
-
Same above
+
:'''Materials , Procedure'''
-
'''Materials , Procedure'''
+
:Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html>
-
Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html>
+
<br/>

Latest revision as of 21:45, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member
NEX , bambi75 and watachin
Materials,Procedure
Same protocol on August 16th.
PCR Product of insert are bcsA and bcsC.

Experiment:PCR

Member
NEX , bambi75 and watachin
Material
  • sterilized water 213μl
  • Ex taq buffer 30μl
  • dNTP 24μl
  • Ex taq 3μl
  • bcsA sense 15μl
  • bcsA antisense 15μl
Procedure
Please see protocol3

Experiment:Restriction enzyme digestion

Member
Same above
Materials
  • PCR production
  • M buffer 30μl
  • BSA 30μl
  • Xba1 6μl
  • Spe1 6μl
Procedure
Please see protocol7

Experiment:Transformation

Member
Same above
Materials , Procedure
Please refer to protocol9

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/08/24"