Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23

From 2010.igem.org

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==2010/8/23 Monday (watachin)==
==2010/8/23 Monday (watachin)==
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===Experiment:Direct PCR(K12-bcsA,K12-bcsC)===
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===Experiment:PCR===
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'''Member'''<br />
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:'''Member'''<br />
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Bambi75 and watachin
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:Bambi75 and watachin
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'''Materials'''
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:'''Materials'''
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*E.coli K12 colony
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:*''E.coli'' K12 colony
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*10µM/l forward Primer 5µl(K12-bcsA,K12-bcsC)
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:*10µM/l forward Primer 5µl(bcsA,bcsC)
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*10µM/l reverse Primer 5µl(K12-bcsA,K12-bcsC)
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:*10µM/l reverse Primer 5µl(bcsA,bcsC)
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*10×EX taq buffer 10µl
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:*10×EX taq buffer 10µl
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*2.5mM each dNTP mixture 10µl
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:*2.5mM each dNTP mixture 10µl
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*EX taq polymerase 1µl
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:*EX taq polymerase 1µl
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*milli-Q 71µl
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:*milli-Q 71µl
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:'''Procedure'''
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'''Procedure'''
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol3</a></html>
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===Experiment:Restriction enzyme digestion===
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:'''Member'''<br />
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:Bambi75 and watachin
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:'''Material'''
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:*bcsA
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:*bcsC
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:You can see other material at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Restriction enzyme digestion">protocol7</a></html>
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===Experiment:Making media(LB broth + amp)===
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:'''Procedure'''
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'''Member'''<br />
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Bambi75 and watachin
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'''Material'''
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Restriction enzyme digestion">protocol7</a></html>
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*LB media 200ml
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*Distilled water 200ml
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*LB Broth 4g
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*ampicillin (50mg/ml) 200μl
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<br/>
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===Experiment:Restriction enzyme digestion===
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'''Member'''<br />
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Bambi75 and watachin
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Latest revision as of 21:42, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/8/23 Monday (watachin)

Experiment:PCR

Member
Bambi75 and watachin
Materials
  • E.coli K12 colony
  • 10µM/l forward Primer 5µl(bcsA,bcsC)
  • 10µM/l reverse Primer 5µl(bcsA,bcsC)
  • 10×EX taq buffer 10µl
  • 2.5mM each dNTP mixture 10µl
  • EX taq polymerase 1µl
  • milli-Q 71µl
Procedure
see protocol3

Experiment:Restriction enzyme digestion

Member
Bambi75 and watachin
Material
  • bcsA
  • bcsC
You can see other material at protocol7
Procedure
see protocol7

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