Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09

(Difference between revisions)

Latest revision as of 06:41, 27 October 2010

E.coli Fiber Project Notebook

Member

naoto and NEX

Material

Procedure

Member

naoto and NEX

Material

we show other material at protocol4

Procedure

see protocol4

@@ Line 2: / Line 2: @@
 ==2010/09/09 Thursday (Naoto)==
 ===Experiment:Ligation===
-'''member'''
+:'''Member'''
-naoto and NEX
+:naoto and NEX
-'''material'''
+:'''Material'''
-*bcsA,B(from ''A.xylinum'') 10µl each
+:*bcsA,B 10µl each
-*bcsC,D(from ''A.xylinum'') 10µl each
+:*bcsC,D 10µl each
-*bcsA,B(from ''E.coli'') 10µl each
+:*bcsA,B 10µl each
-*ligation Mix(nippon gene) 20µl each
+:*ligation Covinience Kit 20µl each
-'''procedure'''
+:'''Procedure'''
-#add material to PCR tube
+:#Add material to PCR tube
-#incubate (20℃　10min)
+:#Incubate (20℃　10min)
 ===Experiment:Electrophoresis===
-'''member'''
+:'''Member'''
-naoto and NEX
+:naoto and NEX
-'''material'''
+:'''Material'''
-*ligation products(see above)
+:*ligation products(see above)
-we show other material at protocol4
+:we show other material at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-'''procedure'''
+:'''Procedure'''
-follow protocol4
+:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
+<br/>