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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/31
From 2010.igem.org
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| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
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=2010/08/31= | =2010/08/31= | ||
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＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR productions | ・PCR productions | ||
| - | *1L(8/25) 8μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L ](8/25) 8μl |
| - | *2L(8/26) 8μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L ](8/26) 8μl |
| - | *4H(8/26) 16μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H ](8/26) 16μl |
| - | *5H(8/26) 16μl | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H ](8/26) 16μl |
・Digest enzyme | ・Digest enzyme | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1+2(8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2 ](8/31) |
| - | *4+5(8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5 ](8/31) |
(after digestion) | (after digestion) | ||
| Line 128: | Line 129: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1+2(8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2 ](8/31) |
| - | *4+5(8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5 ](8/31) |
(After electrophoresis) | (After electrophoresis) | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
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==8：DNA Ligation == | ==8：DNA Ligation == | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1L(8/25) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1L ](8/25) |
| - | *2L(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2L](8/26) |
| - | *4H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4H](8/26) |
| - | *5H(8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5H](8/26) |
(after digestion) | (after digestion) | ||
| Line 156: | Line 156: | ||
We ligated 1 and 2, 4 and 5. | We ligated 1 and 2, 4 and 5. | ||
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==9：Electrophoresis== | ==9：Electrophoresis== | ||
| Line 164: | Line 163: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1+2(8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1+2](8/31) |
| - | *4+5(8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4+5](8/31) |
(After ligation) | (After ligation) | ||
Latest revision as of 18:13, 24 October 2010
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Contents |
2010/08/31
1：Electrophoresis
＜Member＞
Hitomi
＜Sample＞
PCR products.
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
2：PCR
＜Member＞
nito
＜Sample＞
・E-coli (having BBa_I13521 plasmid)
・E-coli (having BBa_K208017 plasmid)
・E-coli (having BBa_I732901 plasmid)
＜Protocol＞
See Protocol 2
・Tube (temperature in annealing)
- Promoter~signal (72.0℃)
- cyaA (71.5)
- mRFP~Terminator (69.0)
- Promoter (70.0)
- RBS~signal (70.0)
- lacZ (63.5)
- Terminator (67.5)
- CRP (72.5)
All tubes ×3. Total 24 tubes.
3：DNA Digestion
＜Member＞
Mariko, Hitomi
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- AvrⅡ
- NheⅠ
- SpeⅠ
＜Protocol＞
See Protocol 9
4：Electrophoresis
＜Member＞
nito, Hitomi
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
5：Electrophoresis
＜Member＞
Mariko, nito
＜Sample＞
(after digestion)
＜Protocol＞
SeeProtocol 8
And cut off gels included DNA.
6：DNA extraction
＜Member＞
nito
＜Sample＞
・PCR productions (8/31)
＜Protocol＞
SeeProtocol 4
7：DNA extraction from gels
＜Member＞
nito
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
8：DNA Ligation
＜Member＞
nito
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 3
We ligated 1 and 2, 4 and 5.
9：Electrophoresis
＜Member＞
nito
＜Sample＞
(After ligation)
＜Protocol＞
SeeProtocol 8
＜Result＞
.JPG)
