Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/27

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/9/27 Monday (watachin)(bambi75)==
==2010/9/27 Monday (watachin)(bambi75)==
-
==PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)==
+
===Experiment:PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)===
-
===member===
+
:'''Member'''
-
watachin and bambi75
+
:watachin and bambi75
-
===materials,Procedure===
+
:'''Materials,Procedure'''
-
Same on August 23th.
+
:Same on August 23th.
-
*95℃5min
+
:*95℃5min
-
*95℃30sec
+
:*95℃30sec
-
*55℃30sec
+
:*55℃30sec
-
*72℃4min
+
:*72℃4min
-
*72℃5min
+
:*72℃5min
-
*10℃∞
+
:*10℃∞
-
（95℃～72℃/30cycle）
+
:（95℃～72℃/30cycle）
-
==Agarose Gels Electro-phoresis==
+
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
-
===member===
+
===Experiment:Agarose Gels Electrophoresis===
-
Same above
+
-
===Materials and Procedure===
+
-
Same protocol on August 16th.
+
-
PCR Product of insert is the above things.
+
-
==restriction enzyme digestion==
+
:'''Member'''
-
===member===
+
:Same above
-
Same above
+
-
===Materials and Procedure===
+
-
Same protocol on August 13rd.
+
-
==Agarose Gels Electro-phoresis==
+
:'''Materials and Procedure'''
-
===member===
+
:Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80agarose gel electrophoresis">the protocol4</a></html>
-
Same above
+
-
===Materials and Procedure===
+
-
Same protocol on August 16th.
+
-
PCR Product of insert is the above things.
+
-
==Ligation==
+
:PCR Product of insert is the above things.
-
===member===
+
===Experiment:Restriction enzyme digestion===
-
Same above
+
-
===materials and procedure===
+
-
Follow the protocol(Ligation).
+
-
*bcsB(E.coli)
+
-
*bcsC(E.coli)
+
-
==Ligation and Transformation==
+
:'''Member'''
-
===member===
+
:Same above  
-
Same above
+
-
===materials and procedure===
+
:'''Materials and Procedure'''
-
Same protocol on September 21st.
+
-
==PCR/bcsQ and bcsEFG(E.coli)==
+
:Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80restriction enzyme digestion">the protocol6</a></html>
-
===member===
+
===Experiment:Agarose gel Electrophoresis===
-
watachin and bambi75
+
-
===materials,Procedure===
+
:'''Member'''
-
Same on August 23th.
+
-
*95℃5min
+
-
*95℃30sec
+
-
*55℃30sec
+
-
*72℃4min
+
-
*72℃5min
+
-
*10℃∞
+
-
（95℃～72℃/30cycle）
+
-
==Agarose Gels Electro-phoresis==
+
:Same above
-
===member===
+
:'''Materials and Procedure'''
-
Same above
+
-
===Materials and Procedure===
+
-
Same protocol on August 16th.
+
-
PCR Product of insert is the above things.
+
 +
:Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
 +
:PCR Production is the above things.
 +
===Experiment:Ligation===
-
===PCR===
+
:'''Member'''
-
PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
+
-
===Electrophoreses of PCR production===
+
-
'''Result'''
+
-
[[Image:2010-09-27-01.jpg]]
+
:Same above
-
===Digestion===
+
:'''Materials and Procedure'''
-
bcsA → Xba,Spe
+
:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
 +
:*bcsB(E.coli)
 +
:*bcsC(E.coli)
-
bcsB → Xba
+
===Experiment:Ligation and Transformation===
-
bcsC → Spe
+
:'''Member'''
-
pSB1C3 → Xba, Spe
+
:Same above
-
===Electrophoreses of PCR productions===
+
:'''Materials and Procedure'''
-
'''Result'''
+
:Same protocol on September 21st.
-
[[Image:2010-09-27-02.jpg]]
+
===Experimet:PCR/bcsQ and bcsEFG(E.coli)===
 +
:'''Member'''
-
===DNA Purification===
+
:watachin and bambi75
-
===Ligation===
+
:'''Materials,Procedure'''
 +
:Same on August 23th.
 +
:*95℃5min
 +
:*95℃30sec
 +
:*55℃30sec
 +
:*72℃4min
 +
:*72℃5min
 +
:*10℃∞
 +
:（95℃～72℃/30cycle）
-
===DNA Purification===
+
===Experiment:Agarose Gels Electrophoresis===
-
===Ligation===
+
:'''Member'''
-
===Transfomation===
+
:Same above
-
===PCR===
+
:'''Materials and Procedure'''
-
===Electrophoreses of PCR productions===
+
:Same protocol on August 16th.
 +
:PCR Product of insert is the above things.
 +
 
 +
===Experiment:PCR===
 +
:'''Member'''
 +
:watachin and bambi75
 +
 
 +
:'''Materials,Procedure'''
 +
 
 +
:PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
 +
:Same on August 23th.
 +
:*95℃5min
 +
:*95℃30sec
 +
:*55℃30sec
 +
:*72℃4min
 +
:*72℃5min
 +
:*10℃∞
 +
:（95℃～72℃/30cycle）
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 +
 
 +
===Experiment:Electrophoreses of PCR production===
 +
 
 +
:'''Member'''
 +
 
 +
:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
 
 +
:Same protocol on August 16th.
 +
:PCR Product of insert is the above things.
 +
 
 +
:'''Result'''
 +
 
 +
:[[Image:2010-09-27-01.jpg]]
 +
 
 +
===Experiment:Digestion===
 +
 
 +
:bcsA → XbaI,SpeI
 +
 
 +
:bcsB → XbaI
 +
 
 +
:bcsC → SpeI
 +
 
 +
:pSB1C3 → XbaI, SpeI
 +
 
 +
===Experiment:Electrophoreses of PCR productions===
 +
 
 +
:'''Member'''
 +
 
 +
:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
 
 +
:Same protocol on August 16th.
 +
:PCR Product of insert is the above things.
 +
 
 +
:'''Result'''
 +
 
 +
:[[Image:2010-09-27-02.jpg]]
 +
 
 +
===Experiment:DNA Purification===
 +
:'''member'''
 +
 
 +
:naoto and watachin
 +
 
 +
:'''procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
 +
 
 +
===Experiment:Ligation===
 +
:'''Member'''
 +
 
 +
:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
 +
 
 +
===Experiment:DNA Purification===
 +
:'''member'''
 +
 
 +
:naoto and watachin
 +
 
 +
:'''procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
 +
 
 +
===Experiment:Ligation===
 +
:'''Member'''
 +
 
 +
:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
 +
 
 +
===Experiment:Transfomation===
 +
 
 +
:'''Member'''
 +
:watachin
 +
 
 +
:'''Materials,Procedure'''
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol9:Transformation protocol9]
 +
 
 +
===Experiment:PCR===
 +
:'''Member'''
 +
:watachin
 +
 
 +
:'''Materials,Procedure'''
 +
:Same on August 23th.
 +
:*95℃5min
 +
:*95℃30sec
 +
:*55℃30sec
 +
:*72℃4min
 +
:*72℃5min
 +
:*10℃∞
 +
:（95℃～72℃/30cycle）
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 +
 
 +
===Experiment:Electrophoreses of PCR productions===
 +
:'''Member'''
 +
 
 +
:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
 
 +
:Same protocol on August 16th.
 +
:PCR Product of insert is the above things.
'''Result'''
'''Result'''
-
[[Image:2010-09-27-03.jpg]]
+
:[[Image:2010-09-27-03.jpg]]
 +
 
 +
<br/>

Latest revision as of 16:55, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
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19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/9/27 Monday (watachin)(bambi75)

Experiment:PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)

Member
watachin and bambi75
Materials,Procedure
Same on August 23th.
  • 95℃5min
  • 95℃30sec
  • 55℃30sec
  • 72℃4min
  • 72℃5min
  • 10℃∞
（95℃～72℃/30cycle）
see protocol3

Experiment:Agarose Gels Electrophoresis

Member
Same above
Materials and Procedure
Same protocol the protocol4
PCR Product of insert is the above things.

Experiment:Restriction enzyme digestion

Member
Same above
Materials and Procedure
Same protocol the protocol6

Experiment:Agarose gel Electrophoresis

Member
Same above
Materials and Procedure
Same protocol4
PCR Production is the above things.

Experiment:Ligation

Member
Same above
Materials and Procedure
Follow protocol8(Ligation).
  • bcsB(E.coli)
  • bcsC(E.coli)

Experiment:Ligation and Transformation

Member
Same above
Materials and Procedure
Same protocol on September 21st.

Experimet:PCR/bcsQ and bcsEFG(E.coli)

Member
watachin and bambi75
Materials,Procedure
Same on August 23th.
  • 95℃5min
  • 95℃30sec
  • 55℃30sec
  • 72℃4min
  • 72℃5min
  • 10℃∞
（95℃～72℃/30cycle）

Experiment:Agarose Gels Electrophoresis

Member
Same above
Materials and Procedure
Same protocol on August 16th.
PCR Product of insert is the above things.

Experiment:PCR

Member
watachin and bambi75
Materials,Procedure
PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
Same on August 23th.
  • 95℃5min
  • 95℃30sec
  • 55℃30sec
  • 72℃4min
  • 72℃5min
  • 10℃∞
（95℃～72℃/30cycle）
see protocol3

Experiment:Electrophoreses of PCR production

Member
Same above
Materials and Procedure
Same protocol on August 16th.
PCR Product of insert is the above things.
Result
2010-09-27-01.jpg

Experiment:Digestion

bcsA → XbaI,SpeI
bcsB → XbaI
bcsC → SpeI
pSB1C3 → XbaI, SpeI

Experiment:Electrophoreses of PCR productions

Member
Same above
Materials and Procedure
Same protocol on August 16th.
PCR Product of insert is the above things.
Result
2010-09-27-02.jpg

Experiment:DNA Purification

member
naoto and watachin
procedure
see protocol6

Experiment:Ligation

Member
Same above
Materials and Procedure
Follow protocol8(Ligation).

Experiment:DNA Purification

member
naoto and watachin
procedure
see protocol6

Experiment:Ligation

Member
Same above
Materials and Procedure
Follow protocol8(Ligation).

Experiment:Transfomation

Member
watachin
Materials,Procedure
see protocol9

Experiment:PCR

Member
watachin
Materials,Procedure
Same on August 23th.
  • 95℃5min
  • 95℃30sec
  • 55℃30sec
  • 72℃4min
  • 72℃5min
  • 10℃∞
（95℃～72℃/30cycle）
see protocol3

Experiment:Electrophoreses of PCR productions

Member
Same above
Materials and Procedure
Same protocol on August 16th.
PCR Product of insert is the above things.


Result

2010-09-27-03.jpg

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/27"