Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/10
From 2010.igem.org
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | ||
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==2010/10/10 Sunday (Naoto)== | ==2010/10/10 Sunday (Naoto)== | ||
- | '''member''' | + | :'''member''' |
- | watachin | + | :watachin |
===Preparation:Measure DNA concentration=== | ===Preparation:Measure DNA concentration=== | ||
- | '''material''' | + | :'''material''' |
- | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
- | '''material'' | + | :'''material''' |
- | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8 | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
- | '''material''' | + | :'''material''' |
+ | |||
+ | :*Competent Cell (Novablue) | ||
+ | :*bcsA in pSB1C3 | ||
+ | :*bcsB in pSB1C3 | ||
+ | :*bcsC in pSB1C3 | ||
+ | :*bcsD in pSB1C3 | ||
+ | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html> | ||
- | + | :'''procedure''' | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol9</a></html> | |
- | + | <br/> |
Latest revision as of 18:10, 27 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/10/10 Sunday (Naoto)
- member
- watachin
Preparation:Measure DNA concentration
- material
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Ligation
- material
- you can see materials at protocol8
- procedure
- refer to protocol8
Experiment:Transformation
- material
- Competent Cell (Novablue)
- bcsA in pSB1C3
- bcsB in pSB1C3
- bcsC in pSB1C3
- bcsD in pSB1C3
- you can see other materials at protocol9
- procedure
- refer to protocol9