Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/20

From 2010.igem.org

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==2010/10/20 Wednesday (Naoto)==
==2010/10/20 Wednesday (Naoto)==
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'''member'''
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:'''member'''
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naoto and watachin
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===Experiment:Sequence===
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:naoto and watachin
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'''material'''
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*PCR
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===Experiment:Colony PCR===
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**bcsD in pSB1C3(No.89~96)
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:'''material'''
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**Big Dye
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**primer
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**DW
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*Ethanol precipitation
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**ethanol
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**EDTA
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'''procedure'''
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:*bcsB:1~24
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:*bcsC:25~48
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:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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#mix materials(DNA<50ng)
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:'''procedure'''
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#PCR
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#Add EDTA and Ethanol and put at room temperature　(15min)
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#centrifuge (8000rpm,30min)
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#throw away supernatant
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#add ethanol again
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#centrifuge (8000rpm,15min)
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#put at refrigerator
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#throw away supernatant
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#add Hi-Di solution
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#transfer these sample to plate for sequence
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#read sequence
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'''result'''
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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Failure to read sequence
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===Experiment:Electrophoresis===
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:'''material'''
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:*PCR production (Colony PCR)
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:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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:'''procedure'''
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:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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:'''result'''
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:We couldn't see bands of bcsB
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:but bcsC were appreard
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<br/>

Latest revision as of 18:03, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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31

2010/10/20 Wednesday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • bcsB:1~24
  • bcsC:25~48
you can see other materials at protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procedure
refer to protocol4
result
We couldn't see bands of bcsB
but bcsC were appreard

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/20"