Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/19

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Notebook/Fiber}})
 
(3 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
 +
__NOTOC__
 +
==2010/10/19 Tuesday (Naoto)==
 +
:'''member'''
 +
:naoto and watachin
 +
 +
===Experiment:Colony PCR===
 +
:'''material'''
 +
 +
:*colony of E.coli
 +
:**bcsB:1~96
 +
:**bcsC:97~196
 +
:other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
:'''procedure'''
 +
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 +
===Experiment:Electrophoresis===
 +
:'''material'''
 +
 +
:*PCR production (Colony PCR)
 +
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
:'''procedure'''
 +
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
 +
 +
:'''result'''
 +
 +
:We couldn't see bands of bcsB and bcsC...
 +
 +
===Experiment:Sequence===
 +
:'''material'''
 +
 +
:*bcsD in pSB1C3(No.89~96)
 +
:*Big Dye
 +
:*primer
 +
:*DW
 +
:*ethanol
 +
:*EDTA
 +
 +
:'''procedure'''
 +
 +
:#mix materials(DNA<50ng)
 +
:#PCR
 +
:#Add EDTA and Ethanol and put at room temperature　(15min)
 +
:#centrifuge (8000rpm,30min)
 +
:#throw away supernatant
 +
:#add ethanol again
 +
:#centrifuge (8000rpm,15min)
 +
:#put at refrigerator
 +
:#throw away supernatant
 +
:#add Hi-Di solution
 +
:#transfer these sample to plate for sequence
 +
:#read sequence
 +
 +
:'''result'''
 +
 +
:Failure to read sequence
 +
 +
<br/>

Latest revision as of 18:02, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/19 Tuesday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • colony of E.coli
    • bcsB:1~96
    • bcsC:97~196
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procedure
refer to protocol4
result
We couldn't see bands of bcsB and bcsC...

Experiment:Sequence

material
  • bcsD in pSB1C3(No.89~96)
  • Big Dye
  • primer
  • DW
  • ethanol
  • EDTA
procedure
  1. mix materials(DNA<50ng)
  2. PCR
  3. Add EDTA and Ethanol and put at room temperature　(15min)
  4. centrifuge (8000rpm,30min)
  5. throw away supernatant
  6. add ethanol again
  7. centrifuge (8000rpm,15min)
  8. put at refrigerator
  9. throw away supernatant
  10. add Hi-Di solution
  11. transfer these sample to plate for sequence
  12. read sequence
result
Failure to read sequence

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/19"