Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/18

From 2010.igem.org

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__NOTOC__
__NOTOC__
==2010/10/18 Monday (Naoto)==
==2010/10/18 Monday (Naoto)==
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
===Experiment:Colony PCR===
===Experiment:Colony PCR===
-
'''material'''
+
:'''material'''
-
*colony of E.coli
+
:*colony of E.coli
-
**bcsD:1~96
+
:**bcsD:1~96
-
other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
:other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-
'''procedure'''
+
:'''procedure'''
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''material'''
+
:'''material'''
-
*PCR production (Colony PCR)
+
:*PCR production (Colony PCR)
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
'''result'''
+
:'''result'''
-
We could see bands of bcsD No.89~96 slightly
+
:We could see bands of bcsD No.89~96 slightly
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''material'''
+
:'''material'''
-
*PCR production (bcsB,bcsC)
+
:*PCR production (bcsB,bcsC and bcsD)
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
'''result'''
+
:'''result'''
-
We could see bands of bcsB and bcsC clearly
+
:We could see bands of bcsB,bcsC and bcsD clearly
<br/>
<br/>

Latest revision as of 18:01, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/18 Monday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • colony of E.coli
    • bcsD:1~96
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procedure
refer to protocol4
result
We could see bands of bcsD No.89~96 slightly

Experiment:Electrophoresis

material
  • PCR production (bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
refer to protocol4
result
We could see bands of bcsB,bcsC and bcsD clearly

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