Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/17

From 2010.igem.org

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__NOTOC__
__NOTOC__
==2010/10/17 Sunday (Naoto)==
==2010/10/17 Sunday (Naoto)==
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'''member'''
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:'''member'''
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naoto and watachin
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:naoto and watachin
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===Experiment:Digestion===
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===Experiment:Colony PCR===
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'''material'''
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:'''material'''
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*bcsD
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*XbaI
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*SpeI
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-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
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'''procedure'''
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:*colony of E.coli
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
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:**bcsD:1~96
 +
:other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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===Experiment:DNA purification with silica gel===
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:'''procedure'''
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'''material'''
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*PCR production(bcsD)
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you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
-
'''procedure'''
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
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===Experiment:Electrophoresis===
 +
:'''material'''
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===Experiment:Ligation===
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:*PCR production (Colony PCR)
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'''material'''
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:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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*bcsD
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*pSB1C3
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you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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===Experiment:Transformation===
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:'''result'''
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'''material'''
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*Competent cell(NovaBlue)
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*bcsD in pSB1C3
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-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
-
'''procedure'''
+
:We couldn't see bands of bcsD...
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
 
 +
<br/>

Latest revision as of 18:00, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/10/17 Sunday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • colony of E.coli
    • bcsD:1~96
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procedure
refer to protocol4
result
We couldn't see bands of bcsD...

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