Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/17

From 2010.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 2: Line 2:
__NOTOC__
__NOTOC__
==2010/10/17 Sunday (Naoto)==
==2010/10/17 Sunday (Naoto)==
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
 +
:naoto and watachin
-
===Experiment:Digestion===
+
===Experiment:Colony PCR===
-
'''material'''
+
:'''material'''
-
*bcsD
+
-
*XbaI
+
-
*SpeI
+
-
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
-
'''procedure'''
+
:*colony of E.coli
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+
:**bcsD:1~96
 +
:other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-
===Experiment:DNA purification with silica gel===
+
:'''procedure'''
-
'''material'''
+
-
*PCR production(bcsD)
+
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
-
'''procedure'''
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+
===Experiment:Electrophoresis===
 +
:'''material'''
-
===Experiment:Ligation===
+
:*PCR production (Colony PCR)
-
'''material'''
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
*bcsD
+
-
*pSB1C3
+
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
-
'''procedure'''
+
:'''procedure'''
-
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
-
===Experiment:Transformation===
+
:'''result'''
-
'''material'''
+
-
*Competent cell(NovaBlue)
+
-
*bcsD in pSB1C3
+
-
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
-
'''procedure'''
+
:We couldn't see bands of bcsD...
-
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
+
 
 +
<br/>

Latest revision as of 18:00, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/17 Sunday (Naoto)

member
naoto and watachin

Experiment:Colony PCR

material
  • colony of E.coli
    • bcsD:1~96
other materials were same as protocol3
procedure
see protocol3

Experiment:Electrophoresis

material
  • PCR production (Colony PCR)
you can see other materials at protocol4
procedure
refer to protocol4
result
We couldn't see bands of bcsD...

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/17"