Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/16

From 2010.igem.org

(Difference between revisions)

Latest revision as of 17:59, 27 October 2010

E.coli Fiber Project Notebook

2010/10/16 Saturday (Naoto)

member

naoto and watachin

Experiment:Electrophoresis

material

PCR production (bcsA,bcsB,bcsC and bcsD)

you can see other materials at protocol4

procedure

see protocol4

Experiment:Digestion

material

bcsD
XbaI
SpeI

you can see other materials at protocol6

procedure

see protocol6

Experiment:DNA purification with silica gel

material

PCR production(bcsD)

you can see materials at protocol5-2

procedure

refer to protocol5-2

Experiment:Ligation

material

bcsD
pSB1C3

you can see materials at protocol7

procedure

refer to protocol7

Experiment:Transformation

material

Competent cell(NovaBlue)
bcsD in pSB1C3

you can see materials at protocol8

procedure

see protocol8

@@ Line 1: / Line 1: @@
 {{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
+__NOTOC__
 ==2010/10/16 Saturday (Naoto)==
-'''member'''
+:'''member'''
-naoto and watachin
+:naoto and watachin
 ===Experiment:Electrophoresis===
-'''material'''
+:'''material'''
-*PCR production (bcsA,bcsB,bcsC and bcsD)
+:*PCR production (bcsA,bcsB,bcsC and bcsD)
-you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
-'''procedure'''
+:'''procedure'''
-see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
+===Experiment:Digestion===
+:'''material'''
+:*bcsD
+:*XbaI
+:*SpeI
+:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
+:'''procedure'''
+:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
 ===Experiment:DNA purification with silica gel===
-'''material'''
+:'''material'''
-*PCR production(bcsD and pSB1C3)
+:*PCR production(bcsD)
-you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+:'''procedure'''
+:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
+===Experiment:Ligation===
+:'''material'''
+:*bcsD
+:*pSB1C3
+:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+:'''procedure'''
+:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
+===Experiment:Transformation===
+:'''material'''
+:*Competent cell(NovaBlue)
+:*bcsD in pSB1C3
+:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
-'''procedure'''
+:'''procedure'''
+:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
-refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
 <br/>