Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/16

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/10/16 Saturday (Naoto)==
 +
:'''member'''
 +
:naoto and watachin
 +
 +
===Experiment:Electrophoresis===
 +
:'''material'''
 +
:*PCR production (bcsA,bcsB,bcsC and bcsD)
 +
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
 +
 +
:'''procedure'''
 +
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html>
 +
 +
===Experiment:Digestion===
 +
:'''material'''
 +
:*bcsD
 +
:*XbaI
 +
:*SpeI
 +
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
 +
 +
:'''procedure'''
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
 +
 +
===Experiment:DNA purification with silica gel===
 +
:'''material'''
 +
:*PCR production(bcsD)
 +
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
 +
 +
:'''procedure'''
 +
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
 +
 +
===Experiment:Ligation===
 +
:'''material'''
 +
:*bcsD
 +
:*pSB1C3
 +
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
 +
 +
:'''procedure'''
 +
 +
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
 +
 +
===Experiment:Transformation===
 +
:'''material'''
 +
:*Competent cell(NovaBlue)
 +
:*bcsD in pSB1C3
 +
:you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
 +
 +
:'''procedure'''
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
 +
 +
 +
<br/>

Latest revision as of 17:59, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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31

2010/10/16 Saturday (Naoto)

member
naoto and watachin

Experiment:Electrophoresis

material
  • PCR production (bcsA,bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
see protocol4

Experiment:Digestion

material
  • bcsD
  • XbaI
  • SpeI
you can see other materials at protocol6
procedure
see protocol6

Experiment:DNA purification with silica gel

material
  • PCR production(bcsD)
you can see materials at protocol5-2
procedure
refer to protocol5-2

Experiment:Ligation

material
  • bcsD
  • pSB1C3
you can see materials at protocol7
procedure
refer to protocol7

Experiment:Transformation

material
  • Competent cell(NovaBlue)
  • bcsD in pSB1C3
you can see materials at protocol8
procedure
see protocol8



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