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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/04
From 2010.igem.org
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===Electrophoreses of PCR production=== | ===Electrophoreses of PCR production=== | ||
| + | :'''Member''' | ||
| + | |||
| + | :Same above | ||
| + | |||
| + | :'''Materials and Procedure''' | ||
| + | |||
| + | :see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4] | ||
| + | |||
:'''Result''' | :'''Result''' | ||
| Line 34: | Line 42: | ||
===Experiment:DNA purification(QIAGEN)=== | ===Experiment:DNA purification(QIAGEN)=== | ||
| + | :'''member''' | ||
| - | + | :naoto and watachin | |
| - | + | :'''procedure''' | |
| - | :''' | + | |
| + | :see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6] | ||
| - | :''' | + | ===Experiment:Ligation=== |
| + | :'''Member''' | ||
| + | :Same above | ||
| - | :''' | + | :'''Materials and Procedure''' |
| + | :Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)]. | ||
| + | |||
| + | ===Experiment:Transformation=== | ||
| + | :'''Member''' | ||
| + | :watachin | ||
| - | :see | + | :'''Materials,Procedure''' |
| + | :see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol9:Transformation protocol9] | ||
Latest revision as of 16:57, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/04 Monday (watachin)
Experiment:PCR
- member
- naoto and watachin
- material
- A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
- If you want to know other materials,refer to protocol3
- procedure
- see protocol3
Experiment:DNA purification(QIAGEN)
- member
- naoto and watachin
- procedure
- see protocol6
Electrophoreses of PCR production
- Member
- Same above
- Materials and Procedure
- see protocol4
- Result
Experiment:DNA purification(QIAGEN)
- member
- naoto and watachin
- procedure
- see protocol6
Experiment:Ligation
- Member
- Same above
- Materials and Procedure
- Follow protocol8(Ligation).
Experiment:Transformation
- Member
- watachin
- Materials,Procedure
- see protocol9
