Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/04

From 2010.igem.org

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===Experiment:PCR===
===Experiment:PCR===
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PCR of A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
+
:'''member'''
 +
 
 +
:naoto and watachin
 +
 
 +
:'''material'''
 +
:A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
 +
 
 +
:If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 +
 
 +
:'''procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 +
 
 +
 
-
Insert check PCR
 
===Experiment:DNA purification(QIAGEN)===
===Experiment:DNA purification(QIAGEN)===
 +
:'''member'''
 +
 +
:naoto and watachin
 +
 +
:'''procedure'''
 +
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
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===Electrophoreses of PCR production===
===Electrophoreses of PCR production===
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'''Result'''
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:'''Member'''
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[[Image:2010-10-04-01.jpg]]
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:Same above
 +
 
 +
:'''Materials and Procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
 +
 
 +
:'''Result'''
 +
 
 +
:[[Image:2010-10-04-01.jpg]]
===Experiment:DNA purification(QIAGEN)===
===Experiment:DNA purification(QIAGEN)===
 +
:'''member'''
-
===Experiment:Ligation===
+
:naoto and watachin
-
===Experiment:Transformation===
+
:'''procedure'''
-
'''member'''
+
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
-
'''material'''
+
===Experiment:Ligation===
 +
:'''Member'''
 +
:Same above
-
'''procedure'''
+
:'''Materials and Procedure'''
 +
:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
 +
 
 +
===Experiment:Transformation===
 +
:'''Member'''
 +
:watachin
-
see protocol8
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:'''Materials,Procedure'''
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol9:Transformation protocol9]

Latest revision as of 16:57, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/04 Monday (watachin)

Experiment:PCR

member
naoto and watachin
material
A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
If you want to know other materials,refer to protocol3
procedure
see protocol3


Experiment:DNA purification(QIAGEN)

member
naoto and watachin
procedure
see protocol6

Electrophoreses of PCR production

Member
Same above
Materials and Procedure
see protocol4
Result
2010-10-04-01.jpg

Experiment:DNA purification(QIAGEN)

member
naoto and watachin
procedure
see protocol6

Experiment:Ligation

Member
Same above
Materials and Procedure
Follow protocol8(Ligation).

Experiment:Transformation

Member
watachin
Materials,Procedure
see protocol9
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/04"