Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/04

From 2010.igem.org

(Difference between revisions)

Latest revision as of 16:57, 27 October 2010

E.coli Fiber Project Notebook

2010/10/04 Monday (watachin)

Experiment:PCR

member

naoto and watachin

material

A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:DNA purification(QIAGEN)

member

naoto and watachin

procedure

see protocol6

Electrophoreses of PCR production

Member

Same above

Materials and Procedure

see protocol4

Result

Experiment:DNA purification(QIAGEN)

member

naoto and watachin

procedure

see protocol6

Experiment:Ligation

Member

Same above

Materials and Procedure

Follow protocol8(Ligation).

Experiment:Transformation

Member

watachin

Materials,Procedure

see protocol9

@@ Line 2: / Line 2: @@
 __NOTOC__
 ==2010/10/04 Monday (watachin)==
-===PCR===
+===Experiment:PCR===
-PCR of A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
+:'''member'''
+:naoto and watachin
+:'''material'''
+:A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
+:If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
+:'''procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
+===Experiment:DNA purification(QIAGEN)===
+:'''member'''
+:naoto and watachin
+:'''procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
-Insert check PCR
-===DNA purification(QIAGEN)===
 ===Electrophoreses of PCR production===
-'''Result'''
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
+:'''Result'''
+:[[Image:2010-10-04-01.jpg]]
+===Experiment:DNA purification(QIAGEN)===
+:'''member'''
+:naoto and watachin
+:'''procedure'''
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
+===Experiment:Ligation===
+:'''Member'''
+:Same above
+:'''Materials and Procedure'''
+:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
-[[Image:2010-10-04-01.jpg]]
+===Experiment:Transformation===
+:'''Member'''
+:watachin
-===DNA purification(QIAGEN)===
+:'''Materials,Procedure'''
-===Ligation===
+:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol9:Transformation protocol9]
-===Transformation===