Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/04

From 2010.igem.org

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__NOTOC__
__NOTOC__
==2010/10/04 Monday (watachin)==
==2010/10/04 Monday (watachin)==
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===PCR===
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===Experiment:PCR===
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PCR of A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
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:'''member'''
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:naoto and watachin
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 +
:'''material'''
 +
:A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
 +
 
 +
:If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 +
 
 +
:'''procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
 +
 
 +
 
 +
 
 +
===Experiment:DNA purification(QIAGEN)===
 +
:'''member'''
 +
 
 +
:naoto and watachin
 +
 
 +
:'''procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
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Insert check PCR
 
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===DNA purification(QIAGEN)===
 
===Electrophoreses of PCR production===
===Electrophoreses of PCR production===
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'''Result'''
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:'''Member'''
 +
 
 +
:Same above
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:'''Materials and Procedure'''
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:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
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 +
:'''Result'''
 +
 
 +
:[[Image:2010-10-04-01.jpg]]
 +
 
 +
===Experiment:DNA purification(QIAGEN)===
 +
:'''member'''
 +
 
 +
:naoto and watachin
 +
 
 +
:'''procedure'''
 +
 
 +
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6]
 +
 
 +
===Experiment:Ligation===
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:'''Member'''
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 +
:Same above
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 +
:'''Materials and Procedure'''
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:Follow [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8(Ligation)].
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[[Image:2010-10-04-01.jpg]]
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===Experiment:Transformation===
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:'''Member'''
 +
:watachin
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===DNA purification(QIAGEN)===
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:'''Materials,Procedure'''
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===Ligation===
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:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol9:Transformation protocol9]
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===Transformation===
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Latest revision as of 16:57, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
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19 20 21 22 23 24 25
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October 2010
SUNMONTUEWEDTHUFRISAT
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/04 Monday (watachin)

Experiment:PCR

member
naoto and watachin
material
A.xylinum bcsA,B,C,D and E.coli bcsA,Q ,pSB1C3
If you want to know other materials,refer to protocol3
procedure
see protocol3


Experiment:DNA purification(QIAGEN)

member
naoto and watachin
procedure
see protocol6

Electrophoreses of PCR production

Member
Same above
Materials and Procedure
see protocol4
Result
2010-10-04-01.jpg

Experiment:DNA purification(QIAGEN)

member
naoto and watachin
procedure
see protocol6

Experiment:Ligation

Member
Same above
Materials and Procedure
Follow protocol8(Ligation).

Experiment:Transformation

Member
watachin
Materials,Procedure
see protocol9