Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/10/03 Sunday (Naoto)==
==2010/10/03 Sunday (Naoto)==
===Experiment:PCR of bcsA('''A.xylinum''')===
===Experiment:PCR of bcsA('''A.xylinum''')===
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
-
'''marerial'''
+
:'''marerial'''
-
*bcsA(''A.xylinum'')
+
:*bcsA(''A.xylinum'')
-
If you want to know other materials, see protocol3
+
:If you want to know other materials, see protocol3
-
'''procedure'''
+
:'''procedure'''
 +
 
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
-
see protocol3
 
===Experiment:Electrophoresis of bcsC,D('''A.xylinum'''),bcsA,B,C,EFG,Q('''E.coli'''),and pSB13===
===Experiment:Electrophoresis of bcsC,D('''A.xylinum'''),bcsA,B,C,EFG,Q('''E.coli'''),and pSB13===
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
-
'''marerial'''
+
:'''marerial'''
-
*bcsC,D(''A.xylinum''),bcsA,B,C,EFG,Q(''E.coli''),pSB13
+
:*bcsC,D(''A.xylinum''),bcsA,B,C,EFG,Q(''E.coli''),pSB13
-
If you want to know other materials, see protocol4
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''procedure'''
 
-
see protocol4
+
:'''procedure'''
 +
 
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
===Experiment:Miniprep of BBa_K145001===
===Experiment:Miniprep of BBa_K145001===
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
-
'''marerial'''
+
:'''marerial'''
-
*Preculture of ''E.coli'' having BBa_K145001
+
:*Preculture of ''E.coli'' having BBa_K145001
-
If you want to know other materials, see protocol9
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html>
-
'''procedure'''
 
-
see protocol9
+
:'''procedure'''
 +
 
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html>
 +
 
===Experiment:Ligation of pSB1C3 and bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
===Experiment:Ligation of pSB1C3 and bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
-
'''marerial'''
+
:'''marerial'''
-
*pSB1C3  
+
:*pSB1C3  
-
*bcsC,D(''A.xylinum''),bcsA(''E.coli'')  
+
:*bcsC,D(''A.xylinum''),bcsA(''E.coli'')  
-
If you want to know other materials, see protocol7
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
-
'''procedure'''
 
-
see protocol7
+
:'''procedure'''
 +
 
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
===Experiment:Transformation of parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
===Experiment:Transformation of parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
-
'''marerial'''
+
:'''marerial'''
-
* parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')
+
:* parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')
-
If you want to know other materials, see protocol8
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
-
'''procedure'''
 
-
see protocol8
+
:'''procedure'''
 +
 
 +
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
 +
 
===Experiment:Electrophoresis of bcsA('''A.xylinum''')===
===Experiment:Electrophoresis of bcsA('''A.xylinum''')===
-
'''member'''
+
:'''member'''
-
naoto and watachin
+
:naoto and watachin
-
'''marerial'''
+
:'''marerial'''
-
*bcsA(''A.xylinum'')
+
:*bcsA(''A.xylinum'')
-
If you want to know other materials, see protocol4
+
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
'''procedure'''
 
-
see protocol4
+
:'''procedure'''
-
'''result'''
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
Band of bcsA could be seen clearly
+
 
 +
:'''result'''
 +
 
 +
:Band of bcsA could be seen clearly
===Experiment:Purification of bcsA('''A.xylinum''')===
===Experiment:Purification of bcsA('''A.xylinum''')===
-
'''member'''
+
:'''member'''
 +
 
 +
:naoto and watachin
 +
 
 +
:'''marerial'''
 +
 
 +
:*bcsA(''A.xylinum'') in agarose gel
 +
:If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
 
 +
 
 +
:'''procedure'''
 +
 
 +
:see protocol4 <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
-
naoto and watachin
 
-
'''marerial'''
 
-
*bcsA(''A.xylinum'') in agarose gel
 
-
If you want to know other materials, see protocol5
 
-
'''procedure'''
 
-
see protocol5
+
<br/>

Latest revision as of 16:42, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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31

2010/10/03 Sunday (Naoto)

Experiment:PCR of bcsA(A.xylinum)

member
naoto and watachin
marerial
  • bcsA(A.xylinum)
If you want to know other materials, see protocol3
procedure
see protocol3


Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13

member
naoto and watachin
marerial
  • bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13
If you want to know other materials, see protocol4


procedure
see protocol4


Experiment:Miniprep of BBa_K145001

member
naoto and watachin
marerial
  • Preculture of E.coli having BBa_K145001
If you want to know other materials, see protocol9


procedure
see protocol9


Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)

member
naoto and watachin
marerial
  • pSB1C3
  • bcsC,D(A.xylinum),bcsA(E.coli)
If you want to know other materials, see protocol7


procedure
see protocol4


Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)

member
naoto and watachin
marerial
  • parts of bcsC,D(A.xylinum),bcsA(E.coli)
If you want to know other materials, see protocol8


procedure
see protocol8


Experiment:Electrophoresis of bcsA(A.xylinum)

member
naoto and watachin
marerial
  • bcsA(A.xylinum)
If you want to know other materials, see protocol4


procedure
see protocol4


result
Band of bcsA could be seen clearly

Experiment:Purification of bcsA(A.xylinum)

member
naoto and watachin
marerial
  • bcsA(A.xylinum) in agarose gel
If you want to know other materials, see protocol5


procedure
see protocol4 protocol4




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